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《Chinese Journal of Clinical Gastroenterology》 2020-02
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Expression of YAP and its effect on proliferation,invasion of colorectal cancer

SHI Wei;NIE Yu-qiang;HE Jie;HUANG Yue-e;ZENG Zheng;Department of Geriatrics,Guangzhou First People's Hospital;Department of Gastroenterology,Guangzhou First People's Hospital;  
[Objective]To investigate the expression of Yes-associated protein(YAP)in colorectal cancer and effect of YAP on the proliferation and invasion of colorectal cancer.[Methods]The difference expression of YAP between colorectal cancer and normal colorectal tissue was studied through bioinformatics analysis.Western blot was used to detect YAP expression in colorectal cancer and normal colorectal tissue.The colorectal cancer cell lines(HCT116 and SW480)with YAP knockdown were constructed;cell proliferation was measured by MTT assay;cell invasion ability was tested through transwell invasion assay.[Results]GSE9348 and GSE20842 two group of microarray data showed that the expression level of YAP mRNA in colorectal cancer was significantly higher than normal colorectal tissue(P0.01).Western blot test results showed that the expression level of YAP protein in colorectal cancer tissues was higher than that in its adjacent normal colorectal tissues(P0.01).MTT dynamic observation showed that the proliferation of colorectal cancer cell lines with YAP knockdown was significantly inhibited compared with the control group(P0.01).The results from transwell assay revealed that the migration cell numbers of colorectal cancer cells with YAP knockdown decreased markedly compared with the control group(P0.01).[Conclusions]The expression of YAP is upregulated in colorectal cancer.YAP can promote the proliferation and invasion ability of colorectal cancer cells.It may be a potential target for treatment of colorectal cancer.
【Fund】: 广东省医学科学技术研究基金项目(No:A2016340);; 广东省省级科技计划项目(No:2016 A020215206)
【CateGory Index】: R735.34
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