Cytotoxicity of T Cell Clones with LAK Activity from Human Cord Blood for Leukemic Cells
Recombinant human interleukine 2 activated mononuclear cells from human cord blood were successfully cloned by using semi-solid medium. About 20% of these clones showed LAK activity against NK sensitive leukemic cell K562, NK resistant liver cancer cell H7402 and multiple leukemic cells including HL-60, KG-1, Jurket and so on. Very high cytotoxicity for those leukemic cells was observed in approximately 50% of LAK clones. Other clones about 80% were not cytotoxic to target cells as mentioned above. The proliferation of HL-60 progenitors was also significantly inhibited by LAK cell clones in vitro.