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《Clinical Medical Journal of China》 2004-03
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Detection Rotavirus Genome in the Blood of Children with Rotavirus Diarrhea by Different Kinds of PCR Tests

Liu Lili 1 Huang Yongkun 2 Wei Qunde 2 , et al. (1. Department of Pediatrics, Beijing General Hospital of Ministry of Coal Industry, Beijing 100028; 2. Department of Pediatrics, The First Affiliated Hospital of Kunming Medical College, Kunming 650032)  
Objective: To investigate the sessitivity of RV RNA with different polymerase chair reaction tests and possibility of viremia in children with rotavirus diarrhea by measuring peripherial blood. Methods: The rotavirus RNA in the plasma samples and peripherial blood mononuclear cells (PBMCs) of 30 patients with rotavirus diarrhea were measured by two kinds of reverse transcriptase polymerase chain reaction (RT-PCR) tests. The positive PCR product was tested by agarose gel electrophoresis and was confirmed by analysing amino acid sequence. Results: One patient was found to be positive for RV RNA in the PBMCs and 30 patients were negative for RV RNA in the plasma. There were no difference between the results of two kinds of RT-PCR tests. The positive PCR product was confirmed as part of human RV VP7 by testing amino acid sequence. Identities of 94% with serotype G1 and 75% with G9 were noted on amino acid sequences. Extraintestinal symptoms were found in the positive case.Conclusion:RV RNA can be detected in the blood of children with rotavirus diarrhea by different kinds of PCR tests. Rotavirus may be transmitted by the PBMCs outside the intestinal tract and occur viremia which induces extraintestinal injury.
【CateGory Index】: R446.5
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