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Association analysis between antioxidant activity and main components of pine pollen

DONG Su-zhe,PEI Jin,LIU Wei,ZHANG Yi-nan,WAN De-guang Pharmacy College,Chengdu University of Traditional Chinese Medicine;The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine;State Key Laboratory Breeding Base of Systematic Research,Development and Utilization of Chinese Medicine Resources,Chengdu 610075,China)  
Objective:To study the antioxidant activities and active extracts of pine pollen.Method:UV spectrophotometry was used to detect the content of total flavonoids,total sterols and total polysaccharides and the activity of scavenging DPPH and hydroxide free radical of pine pollen from different areas.Multivariate correlation analysis method was used to process date.Result:the extracts of pine pollen showed antioxidant activities of scavenging two different free radicals with obvious concentration-scavenging capacity relationship.The IC50 of scavenging DPPH of natural and wall-broken pine pollen were 14.86 g﹒L-1 and 12.95 g﹒L-1,respectively.The IC50 of scavenging hydrogen free radical of natural and wall-broken were 20.31 g﹒L-1 and 3.45 g﹒L-1.The content of total flavonoids,total sterols and total polysaccharides was negatively correlated with the IC50 of scavenging DPPH and hydrogen free radical.The content of total flavonoids showed the maximal relevance and the content of total polysaccharides showed the minimal relevance.Conclusion:Pine pollen show definite antioxidant actions with the total flavonoids and total sterols as the active fraction.the wall breaking can effectively improve the activity of scavenging DPPH and hydrogen free radical and the content of total flavonoids and total sterols.[
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