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《Journal of Environmental & Occupational Medicine》 2007-06
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Application of Computer Assisted Sperm Analysis System in Detection of Rat Sperm Motility

WEI Ling-ya~(1,2) LO Zhong-ming~1 WANG Min-sheng~1 (1.Department of Toxicology,Jiangsu Centers for Diseases Prevention and Control,Nanjing 210009,China;2.School of Public Health,Southeast University,Nanjing 210009,China)  
[Objective]To study the effect of cadmium chloride (CdCl_2) on rat sperm motility in vitro with computer assisted sperm analysis system (CASA).[Methods]The suspension of spermatozoa was obtained from cauda epididymides using"diffusion"method.Different doses of cadmium chloride (0,0.5,1.0,2.0,4.0,8.0,16.0,32.0 mg/L)were administrated to spermatozoa suspension.Sperm motility were evaluated with CASA after 30 min,1 h,2 h,and 4 h of exposure.[Results] After 30 min exposure in CdCl_2,the indices such as VAP,VCL,VSL,LIN and ALH were significantly decreased (P0.05), BCF and STR were decreased significantly in the groups of exposed to 1.0~32.0mg/L CdCl_2.VAP showed relationship with the dose (r=-0.348,P0.05).With the increasing of dose and the exposure continuance of cadmium chloride,sperm motility decreased significantly.[Conclusion]Cadmium chloride could result in toxic effects on motility of sperms.
【Fund】: 江苏省疾病预防控制中心青年科研基金(编号:JSQ200509)
【CateGory Index】: R114
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