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《Physical Testing and Chemical Analysis(Part B:Chemical Analysis)》 2012-07
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UHPLC-MS/MS Determination of Cholesterol in Edible Vegetable Oil

ZHU Rong,ZHANG Yi,YANG Mei,CHEN Hong,QIAO Bin-zong,PU Xu-feng(Chengdu Center of Food and Drug Control,Chengdu 610045,China)  
A method for the determination of cholesterol in edible vegetable oil by ultra-high-performance liquid chromatography hyphenated with tandem mass spectrometry(HPLC-MS/MS) was proposed.Cholesterol in samples was extracted with a mixture of petroleum ether and ethyl ether(1+1).The eluate was used for UHPLC-MS/MS determination,in which the ACQUITY UPLC BEH C18 column(50 mm×2.1 mm,5 μm) was used as separation column,a mixture of formic acid and methanol(0.1+99.9) was used as mobile phase,and 2,2,3,4,4,6-d6-cholesterol was used as internal standard.Atmospheric pressure chemical electrospray ionization under the mode of multiple reaction monitoring was used in the detection.Ion pairs used for the quantitative analysis were m/z 369.2/146.9,369.2/160.9 for cholesterol and m/z 375.2/166.5 for internal standard.Linearity range was found between 0.1-5 mg·L-1,and lower limit of determination(10S/N) found was 0.02 ng.Recovery was tested at 3 concentration levels,giving results of recovery ranged from 102% to 110%.
【CateGory Index】: O657.63;TS227
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