SYNTHESIS AND PROPERTY OF STIGMASTEROL MOLECULAR IMPRINTING POLYMER

HAN Yongping1,2 HE Xuwen1 LIN Qiang2 1.China university of mining and technology,Beijing 100083,China 2.Bio-chemical engineering college of Beijing union university,Beijing 10023,China  

The molecular imprinting polymeric microspheres(MIPs) of stignmasterol was synthesized by suspension polymerization,utilizing stignmasterol(stig.) as template molecule,methyl methacrylate(MMA) as functional monomer,divinyl benzene(DVB) as cross-linker agent,CaCO3 and SDS as dispersant,Na2S2O8 as initiator and lowpolar solvents(toluene) as porogenic solvents.The morphology,averages size and selectivity for stignmasterol of the synthesized polymers were respectively examined and evaluated by HPLC,SEM and Photon Correlation Spectroscopy.The experiment results showed that the optimal condition was 3g stig.:40mL MMA:15mL DVB:4g CaCO3+2g SDS:14mL tolu..During polymerization initiator Na2S2O8 should be added in more batches and in the early period a high speed string was applied,with the polymerization the string rate was reduced.The prepared MIPs,which average diameters were below 100μm and narrow size distribution,could effectively separate stignmasterol from structural analogues β-stiosterol with above 1.65 separate factor and about 1.2mg/g adsorptive capacity for stigmasterol.

【Key Words】： Molecular imprinting polymer Stigmasterol Adsorptive capacity Specific recognition and binding performance
【Fund】： 北京市教委支持项目(KM200511417005)
【CateGory Index】： O631.3

Download(CAJ format) Download(PDF format)

CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.