The Relationship between the Defensive Effect of Chitosan on MDMV and the Activity of Defensive Enzyme in Maize Lesves
JIANG Hua 1, NING Shu xiang 1, YANG Wen xin 1, WANG You fu 2(1.Life College,Liaoning Normal University,Dalian 116029,China;2.Liaoning Entry Exit Inspection and Quarantine Bureau of P.R.,Dalian 116021,China)
The effects of chitosan on the several defensive enzymes in maize leaves infected with MDMV in the artificial friction way were tested by means of colorimetric analysis. The primary results indicate that chitosan is capable of strengthening the activity of enzyme or shifting its peak to earlier time. Especially the activity of PO goes up strikingly while that of PPO, PAl and chitin increases slightly and appears mainly in the first few days of treatment. Thus the anti virus organism induced by chitosan seems to relate to PO, but not or a little to relate to PPO, PAL and chitin. In addition, the examination of medical defensive effect shows that chitosan can prevent diseases to a certain extent.The content of soluble protein in the inoculated maize leaves decreases whereas that in the maize leaves inoculated with virus after being sprayed with chitosan declines a little, which to some degree reveals that chitosan can desist from virus reproduction.