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《Journal of Liaoning Normal University(Natural Science Edition)》 2007-01
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Obtaining FSTs of Nodulisporium sylviforme fusant by TAIL-PCR techniques

CHI Yan1,2,ZHOU Dong-po1,PING Wen-xiang1,LI Shan-shan1,ZHU Jing1(1.Key Laboratory of Microbe,Heilongjiang University,Harbin 150080,China;2.Key Laboratory of Bio-organic Chemistry,Dalian University,Dalian 116622,China)  
TAIL-PCR technique was performed to get the flanking sequence of T-DNA tags(FSTs) from six randomly selected HDF-68 positive T-DNA transformants only in one day time.After analysis of its sequences,the main factors of influencing TAIL-PCR amplification were discussed.This study provided a feasible way to seperate taxol biosynthesis related genes for taxol-producing fungi from T-DNA mutants,and also laid solid foundation for clarifying taxol pathway in taxol-producing fungi and for finally constructing high-yield gene engineering strain.
【Fund】: 国家自然科学基金资助项目(30570025)
【CateGory Index】: Q78
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