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Study on mechanism of receptor-mediated modulation of nitric oxide synthase of vascular endothelial cell

RUAN Yun jun,WU Sai zhu,MENG Su rong et al (Department of Cardiology,Nanfang Hospital,The First Military Medical University,Guanghzhou,Guangdong 510515,China)  
Objective To investigate the effect of 17β estradiol on vascular endothelial nitric oxide synthase (NOS).Methods Rat lung vascular endothelial cells(EC)were cultured according to the blocks explanting method and propagated in phenol red free 1640 medium.The cells were treated with several different concentrations of 17β estradiol (E 2 0.1 nmol-10 nmol) with or without estrogen receptor antagonist tamoxifen (1 nmol/L).The receptor binding assay (RBA) was adopted to measure the estrogen receptors in the endothelial cells.Results (1)Treatment with 17β estradiol for 8 hour to 24 hours significantly enhanced the production of NO (1 nmol/L,10 nmol/L E 2 vs control,P0.05) and NOS activity (10 nmol E 2 vs control,8 h,P0.05;16 h,24 h,P0.01) in EC.(2) RBA showed the existence of estrogen receptors in endothelial cells.(3)Estrogen receptor antagonist tamoxifen significantly inhibited the effect of estradiol (P0.01).Conclusion 17β estradiol could enhance the production of NO and the NOS activity in vascular endothelial cells via receptor mediated way,and estrogen receptor may be important in the release of NO from vascular endothelial cell.The result may contribute to explaining the beneficial effect of estrogen in the prevention of atherosclerosis.
【Fund】: 国家自然科学基金!(以心血管为靶点的雌激素作用及机制研究 基金号 3960 0 1 69);; 广东省自然科学基金!(性激素与动脉粥样硬化发病关系分
【CateGory Index】: R96
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