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《Liaoning Journal of Traditional Chinese Medicine》 2009-08
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Effect of Drug Serum of Compatibility Astragalus and Curcumae on Proliferation and Differentiation of Human Gastric Cancerous Cell Strain MKN45

ZHENG Kai,SHEN Hong (Department of Digestion,Jiangsu Province Hospital of Traditional Chinese Medicine,Nanjing 210029,Jiangsu,China)  
Objective:To observe the effect of drug serum of compatibility Astragalus and Curcumae on proliferation and differentiation of human gastric cancerous cell strain MKN45,compare differences in application of drug combination and individual,and provide experimental evidence for treating gastric cancer by invigorating qi and activating blood.Methods:Preparing drug serum of compatibility Astragalus and Curcumae according to pharmacology of Chinese materia medica,detecting proliferation inhibition rate of MKN45 cells by MTT,and determining the activity of marker enzymes AKP,LDH by an enzyme-labeling instrument.Results:In the MTT experiment,Group Curcumae inhabited cells more powerfully after 48 hous in contrast with Control Group(P0.01).After 72 hours,Group Curcumae and Combination Group still inhibited cell proliferation,displaying the difference between them and Control Group had statistical significance(P0.01).Combination Group stably inhibited MKN45 proliferation after 24 hours,48 hours and 72 hours.By an enzyme-labeling instrument,activity expression of AKP,LDH of MKN45 in Group Curcumae and Combination Group decreased markedly,displaying the difference between them and Control Group had statistical significance(P0.05)and the difference between Combination Group and Group Astragalus had statistical significance(P0.01).Conclusion:Drug serum of compatibility Astragalus and Curcumae inhibited proliferation and induced differentiation of gastric cancerous cell strain MKN45.Comparing with individual drugs,the combination had the advantage of effect stability and synergy,which proves that the principle of invigorating qi and activating blood is effective for treating gastric cancer by TCM.
【Fund】: 江苏省中医药局资助课题(H05044)
【CateGory Index】: R285;R735.2
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