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《Liaoning Journal of Traditional Chinese Medicine》 2011-03
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Correlation between the Cumulative Analgesic Effect of Electroacupuncture and the Expression of Hypothalamic Pre-proopiomelanocortin mRNA and Hypothalamic and Hippocampal Prepronociceptin mRNA in Chronic Neuropathic Pain Rats

WANG Jun-ying,MENG Fan-ying,CHEN Shu-ping,GAO Yong-hui,LIU Jun-ling(Department of Physiology,Institute of Acu-moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China)  
Objective:To investigate the mechanism of repeated electroacupuncture(EA) underlying the cumulative analgesic effect in neuropathic pain rats.Methods:A total of 35 Wistar rats were randomized into control(n=5),chronic constrictive injury(CCI)(n=5),CCI+EA2 day(D)(n=5),ovariectomy(OVX)+CCI(n=5),OVX+CCI+EA2D(n=5),OVX+CCI+EA2 weeks(W)(n=5) groups.The OVX rats were subcutaneously injected with D-galactose(15mg/100g) solution,once daily for 45 days(D).Chronic pain model was established by ligating the left sciatic nerve with pieces of surgical suture.EA(2/15Hz,1~2mA)was applied to bilateral "Zusanli"(ST 36)-"Yanglingquan"(GB 34) for 30 min,once daily for 2D and 2 W,respectively.Hypothalamic Pre-proopiomelanocortin(POMC) mRNA expression,and hypothalamic and hippocampal prepronociceptin(PNOC) mRNA expression were detected by reverse transcription polymerase chain reaction(RT-PCR).Results:In comparison with the normal control group,the hypothalamic POMC mRNA expression was upregulated significantly(P0.05) and hypothalamus and hippocampal PPNOC mRNA expression downregulated considerably in CCI group(P0.05).Compared with the CCI group,the expression of hypothalamic POMC mRNA was increased markedly in CCI+EA2W group(P0.05),while the expression of hippocampal PNOC mRNA decreased distinctly in both CCI+EA2D and CCI+EA2W groups(P0.05).In OVX+CCI rats,compared with the OVX+CCI group,hypothalamic POMC mRNA expression was upregulated significantly in OVX+CCI+EA2W group(P0.05),whereas hippocampal PNOC mRNA expression was downregulated remarkably(P0.05).No significant differences were found between the CCI and CCI+EA2D groups,and between the OVX+CCI and OVX+CCI+EA2D groups in the expression of hypothalamic POMC mRNA;between the CCI+EA2D and CCI+EA2W groups,between the OVX+CCI and OVX+CCI+EA2D groups,and between the OVX+CCI and OVX+CCI+EA2W groups in hypothalamic PPONC,and between OVX+CCI+EA2D and OVX+CCI+EA2W groups in hippocampal PNOC mRNA expression(P0.05).Conclusion:The cumulative analgesic effect of repeated EA of ST36-GB34 is closely related to the upregulation of hypothalamic POMC expression and the downregulation of both hippocampal and hypothalamic PNOC mRNA expression.
【Fund】: 国家自然科学基金资助项目(30472241 30973796 90709031);; 国家重点基础研究发展计划(973计划)资助项目(2007CB512505 2006CB504506 2005CB523306)
【CateGory Index】: R245.97
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