Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Clinical and Experimental Pathology》 2014-09
Add to Favorite Get Latest Update

Comparative study on Wnt,Notch signaling pathway molecules in primary breast cancer and matched lymph nodes metastasis stem cells

ZENG Ling-rui;HAN Yu-zhen;MA Ying-lu;LI Wen-xing;Department of Pathology,Affiliated Hospital of Binzhou Medical College;Department of Pathology,Binzhou Medical University;Department of Thyroid and Breast Surgery,Affiliated Hospital of Binzhou Medical College;  
Purpose To investigate the gene expression and significance of β-catenin,Cyclin D1 and Notch1 in primary breast cancer stem cells( BCSC) and matched lymph node metastasis stem cells. Methods 30 cases of breast invasive ductal carcinoma and matched metastasis lymph nodes were made into single cell suspensions,then BCSC were separated from them by immunomagnetic sorting. β-catenin,Cyclin D1 and Notch1 gene expressions of Wnt,Notch signaling pathway were detected by real time PCR. Results The expression of β-catenin in primary BCSC and matched lymph nodes metastasis stem cells had statistically no differences( P 0. 05),while the expression of Cyclin D1 and Notch1 in matched lymph nodes metastasis stem cells were significantly higher than the expression in primary BCSC( P 0. 01,respectively). Conclusion Compared with the primary cancer stem cells,Cyclin D1 and Notch1 activation in metastasis cancer stem cells are in higher level,which leades to a higher capability of invasion and metastasis,which may be a new therapeutic target.
【Fund】: 山东省自然科学基金(ZR2010HM096)
【CateGory Index】: R737.9
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 5 Hits
1 HUA Ben-lin1,FU Xin-ge2,LI Feng1,YIN Liang1(1Department of Pathology,Shihezi University School of Medicine,Shihezi 832002,China;2Department of Pathology,the First Affiliated Hospital,Shantou University School of Medicine,Shantou 515041,China);Comparison of Notch1 and JAG1 expression in human breast carcinoma and adjacent tissues[J];Chinese Journal of Clinical and Experimental Pathology;2009-02
2 XU Shan-shan1,HAN Yu-zhen1,MA Wen-hao1,ZHU Yu-hong2(1Department of Pathology,Binzhou Medical University,Binzhou 256603,China;2Department of Pathology,Affiliated Hospital of Binzhou Medical University,Binzhou 256603,China);Expression and significance of Hedgehog signaling proteins in primary breast cancer and matched lymph node metastases[J];Chinese Journal of Clinical and Experimental Pathology;2012-11
3 HAO Xi shan *, FENG Yu mei , ZHANG Liang, LI Xiao qing,NIU Yun, XIAO Chun hua, SUN Bao cun. *Cancer Institute & Hospital, Tianjin Medical University, Tianjin 300060, China;Screening of differential expression genes between primary breast cancer and its lymph node metastasis using single primer amplification of cDNA for microarray[J];National Medical Journal of China;2005-06
4 FENG Yu-mei GAO Guang ZHANG Fang CHEN Hui WAN Yan-fang LI Xiao-qing Department of Biochemistry & Molecular Biology,Tianjin Cancer Hospital & Institute,Breast Cancer Prevention and Treatment Key Laborotery of Ministry of Education,Tianjin Medical University,Tianjin 300060,China;Identification of the differentially expressed genes between primary breast cancer and paired lymph node metastasis through combining mRNA differential display and gene microarray[J];National Medical Journal of China;2006-39
5 HAN Yuzhen1,SANG Jing2,LU Zenghua3,ZHU Yuhong3 (1.Department of Pathological Sciences,Binzhou Medical University,Binzhou,Shandong 256603,China;2.Department of Pathology,the Central Hospital of Tai'an,Tai'an,Shandong 271000,China;3.Department of Pathology,the Affiliated Hospital,Binzhou Medical University,Binzhou,Shandong 256603,China);Clinicopathologic significance of breast cancer stem cells concerning their content and expression of drug resistance related proteins[J];Chinese Journal of General Surgery;2012-11
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 L Xin-quan, SUO Zhen-he, MA Chang-lu, ZHAO A-hong, SONG Yi-min, LI Hui-xiang(Department of Pathology, First Affiliated Hospital of Zhengzhou University, the Tumor Pathology Key Laboratory of Henan Province, Zhenzhou 450052, China);Expression of mammary epithelial stem-like cells in breast benign and malignant lesions[J];Chinese Journal of Clinical and Experimental Pathology;2008-04
2 ZHANG Guo-ying, WANG Shu-yu(Department of Obstetrics and Gynecology,the First Affiliated Hospital of NJMU,Nanjing 210029,China );Different expression of a synthetic enzymen for L-selectin ligant in the endometrium from the patients with carcinoma of endometrium and normal women[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2007-07
3 LIN Feng;ZHANG Chen-hong;ZHOU Zhi-jun;LIU Fang;WEI Ruo-han;CHEN Hui;First People's Hospital of Taizhou;Organ Transplantation Center,Changzheng Hospital,Second Military Medical University;;Effect of hepatocellular carcinoma suppressor gene-1 on the cancer stem-like phenotype and invasive and metastatic ability of liver tumor cells[J];Medical Journal of Chinese People's Liberation Army;2014-04
4 ZHAO Chen;Department of Developmental Biology,Stanford University;;Paracrine signaling in stem cell renewal and in neoplastic tumor growth[J];中国科学:生命科学(英文版);2014-06
5 WANG Ming-bo;LIU Jun-ling;GUO Fang;HAN Ya-ling;XIE Xiao-dong;General Hospital of Shenyang Military Area Command,Oncology Diagnosis and Treatment Center;General Hospital of Shenyang Military Area Command,Cardiovascular Diseases Institute of PLA;;A Case-control Study between Multi-line and First-line Internal Medicine of Rescue Therapy for Breast Cancer[J];Medical & Pharmaceutical Journal of Chinese People's Liberation Army;2014-03
6 MA Lei;WU Si-pei;DENG Xu-bin;JIN Chuan;LU Qin;LIU Xiao-yan;CHEN Wen-sheng;ZOU Qing-feng;The Third District of Internal Medicine Department, Affiliated Cancer Hospital of Guangzhou Medical University/Cancer Center of Guangzhou Medical University(CCGMU);Institute of Lung Cancer Research, Guangdong Provincial People's Hospital;;Inhibition of D-CIK on circulating tumor cells in advanced breast cancer[J];Hainan Medical Journal;2014-13
7 KONG Mian;WANG Hong - yue;LIU Zhi - yong;HOU Chuan - qiang;LI Bao - jiang;Dept.of Breast Surgery,the Central Hospital of Taian;;Screen peptides specifically bonding to breast cancer primary cells[J];Journal of Taishan Medical College;2013-12
8 KONG Mian;DAI Shu-qin;LI Bao-jiang;WANG Jun-ye;LIN Yu;ZANG Lin-quan;CAO Kai-yuan;State Key Laboratory of Oncology in Southern China,Sun Yat-sen University Cancer Center;Department of Breast Surgery,Central Hospital of Taian;Department of Pharmacology,College of Pharmacy,Guangdong Pharmaceutical University;Department of Microbiology,Sun Yat-sen University;;Isolation of breast cancer stem cell and screening of specific polypeptide bonding to it[J];Chinese Journal of Cancer Prevention and Treatment;2013-24
9 LIU Yan-ju,LI Dong-hui,CAO Ming-yao.Medical College of Hebei University of Engineering,Handan,056029.;EXPRESSION OF C-ERBB-2,P52 AND PCNA IN BREAST CANCER[J];Modern Preventive Medicine;2006-12
10 ZHANG Guoying,WANG Shuyu.Department of Obstetrics and Gynecology,First Affiliated Hospital,Nanjing Medical University,Nanjing 210029,CHINA;Expression of L-selectin in patients with carcinoma of endometrium[J];Jiangsu Medical Journal;2007-09
【Secondary Citations】
Chinese Journal Full-text Database 7 Hits
1 LI Chuan-wei1,LI Lian-hong2,LIU Ya-ling1,FAN Shu-jun2,WANG Bo2,SUN Jie2(Department of Pathology, Taishan Medical College,Taian 271000, China; 2Department of Pathology, Dalian Medical University, Dalian 116044, China);Role of Notch1 signal in differentiaton of mammary cancer stem cells[J];Chinese Journal of Clinical and Experimental Pathology;2008-02
2 LIN Shuai,WU Cheng-yi(Department of Endocrine Surgery,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China);Distribution and significance of CD44~+/CD24~- phenotype and molecular subtypes of breast cancer in breat carcinoma[J];Chinese Journal of Clinical and Experimental Pathology;2010-03
3 ZHANG Jin-zhi,ZHANG Feng-yan,LIN Zhen-hua,XUAN Yan-hua(Department of Pathology,Yanbian University College of Medicine,Yanji 133000,China);Clinicopathological significance of shh,ptch and smo protein overexpression in breast cancers[J];Chinese Journal of Clinical and Experimental Pathology;2010-05
4 YANG Xiao-wen,CUI Ming,MO Ping. Department of Surgery,Kunming General Hospital of Chengdu Command,Kunming 650032,China;Impact of preoperative chemotherapy on the expressions of GST-π,P-gp and TOPO-Ⅱ in breast cancer tissues[J];Chinese Journal of Breast Disease(Electronic Edition);2009-01
5 ZUO Wen-shu, WEI Ling, SONG Xian-rang,WANG Xing-wu, WANG Ming-yu,WANG Yong-sheng, YU Zhi-yong, BI Ye. First surgical department of Shandong Tumor Hospital, Jinan 250117, China;The clinical significance of P-glycoprotein,lung resistance protein and multidrug resistance related protein expressions in infiltrating breast carcinoma tissues[J];National Medical Journal of China;2006-44
6 YANG Guohua,CHEN Xiaogeng(Department of Oncosurgery,Fujian Province Hospital,Fuzhou 350001,China);Research on correlation factors of breast cancer stem cell and breast cancer metastasis[J];Chinese Journal of General Surgery;2009-05
7 LI Zhi,LIU Chun-ping,HE Yan-li,ZHANG Jing-hui,HUANG Tao (Department of Breast and Thyroid Surgery, Union Hospital,Huazhong University of Science and Technology,Wuhan 430022,China);The expression of multi-drug resistance gene in breast cancer stem cells and its significance[J];Tumor;2008-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved