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《Journal of Clinical Transfusion and Laboratory Medicine》 2006-03
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Recovery of Platelet Count of Platelet Concentrates Following Cryopreservation with Various Doses of Second-messenger Effectors and 2% Dimethyl Sulfoxide as Cryopreserving Solution

GU Hai-hui,QIAN Bao-hua,SONG Yun,et al.Department of Blood Transfusion,Changhai Hospital,Second Military Medical University,Shanghai 200433  
Objective To study the recovery of platelet count of platelets following cryopreservation with various doses of second-messenger effectors(thrombosol:amiloride,sodium nitroprusside,adenosine) and 2% DMSO as cryopreserving solution.Methods Part 1,fresh platelet concentrates were divided into 6 groups,using 2% DMSO,6% DMSO,2% DMSO plus thrombosol and 2% DMSO plus one of the contents of thrombosol as cryopreserving solution.Part 2,fresh platelet concentrates were divided into 10 groups,using 2% DMSO,6% DMSO,2% DMSO plus thrombosol and 2% DMSO plus various doses of amiloride and sodium nitroprusside as cryopreserving solution. After frozen for 1 week or 1 month at -80℃, all groups were thawed and tested for platelet count. Results Part 1,there was no difference between adeno-sinegroupand2%DMSOgroup(P0.05),butthesetwogroupsweresignificantlylowerthanthoseofother4groups (P0.05 or P0.01). Part 2,half dose of amiloride and sodium nitroprusside group was significantly higher than that of 2% DMSO group (P0.01),and had no difference with that of 6% DMSO group or thrombosol group(P0.05).Conclusion 2% DMSO plus half dose of amiloride and sodium nitroprusside as cryoprotective agent have the same effect as 6% DMSO or 2% DMSO plus thrombosol on protection against platelet count decrease of thawed platelet.
【CateGory Index】: R457.1
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【References】
Chinese Journal Full-text Database 1 Hits
1 HU Hui-hong1,QIAN Bao-hua1,LIU Bao-lin2,GU Hai-hui1,ZHU Yan-xia1,HUA Mei-xian1 1.Department of Blood Transfusion,Changhai Hospital,Second Military Medical University,Shanghai 200433,China2.Biotechnology Institute of Low Temperature,University of Shanghai for Science and Technology,Shanghai 200093,China;Influence of cooling rate on quality of cryopreservted platelets[J];Academic Journal of Second Military Medical University;2008-07
【Citations】
Chinese Journal Full-text Database 1 Hits
1 GU Hai-hui,QIAN Bao-hua *, ZHANG Hao-xiang,WU Jiang,WANG Xuan, HUA Mei-xian, GUO Feng (Department of Blood Transfusion,Changhai Hospital , Second Military Medical University,Shanghai 200433,China);Mixture of low concentration dimthyl sulfoxide and second-messenger effector as cryopreserving solution in protection of platelets activity[J];Academic Journal of Second Military Medical University;2005-04
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 HU Hui-hong1,QIAN Bao-hua1,LIU Bao-lin2,GU Hai-hui1,ZHU Yan-xia1,HUA Mei-xian1 1.Department of Blood Transfusion,Changhai Hospital,Second Military Medical University,Shanghai 200433,China2.Biotechnology Institute of Low Temperature,University of Shanghai for Science and Technology,Shanghai 200093,China;Influence of cooling rate on quality of cryopreservted platelets[J];Academic Journal of Second Military Medical University;2008-07
【Co-references】
Chinese Journal Full-text Database 7 Hits
1 Ouyang Xilin,Liu Jinghan,Dayong Gao.PLA General Hospital,Beijing 100853,China;Flow cytometry detection of CD62p reexpression by preserved platelets and it's value in evaluation of the quality of preserved platelets[J];Chinese Journal of Blood Transfusion;2002-02
2 CHEN Linfeng, LIU Jinghan, OUYANG Xilin, et al. Department of Blood Transfusion, Center for Military Clinical Blood Transfusion, Chinese PLA General Hospital, Beijing 100853, China;Effects of trehalose loading on the freeze-dried red blood cells[J];Chinese Journal of Blood Transfusion;2008-01
3 LIU Jinghan,OUYANG Xilin,ZHUANG Yuan,et al.Department of Blood Transfusion Medicine,PLA General Hospital,Beijing 100853,China;The feasibility of platelet long cryopreserved at-80℃[J];Chinese Journal of Blood Transfusion;2008-04
4 GU Hai-hui,QIAN Bao-hua *, ZHANG Hao-xiang,WU Jiang,WANG Xuan, HUA Mei-xian, GUO Feng (Department of Blood Transfusion,Changhai Hospital , Second Military Medical University,Shanghai 200433,China);Mixture of low concentration dimthyl sulfoxide and second-messenger effector as cryopreserving solution in protection of platelets activity[J];Academic Journal of Second Military Medical University;2005-04
5 Liu Jinghan and Han Yufeng.Department of Transfusion,Genefal Hospital of PLA.Li Cifen and Yin Songjian.Department of Laboratory,General Hospital of PLA;USE OF DIMETHYLSULFOXIDE FOR PRESERVATION OF PLATELETS UNDER FROZEN CONDITION[J];Medical Journal of Chinese People's Liberation Army;1989-02
6 Liu Jinghan, Wang qingmei, Li Xijin et al. General Hospital of PLA, Beijing 100853;EFFECT OF CRYOPRESERVED PLATELETS TRANSFUSION ON 1560 PATIENTS[J];Medical Journal of Chinese People's Liberation Army;2001-03
7 LIU Jing Han, OUYANG Xi Lin, Lü Liu Cai, Dayong Gao 1,2 (Department of Blood Transfusion, The General Hospital of PLA, Beijing 100853, China; 1Institute of Cryobiomedical Engineering, Faculty of Thermal Science and Energy Engineering, Chinese Science and Techinology University; 2University of Kentucky, Lexington KY40506 USA);Thermometry of Intracellular Ice Crystal Formation in Cryopreserved Platelets[J];Journal of Experimental Hematology;2002-06
【Secondary Citations】
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1 Zhang Haoxiang1,Qian Baohua1*,Guo Feng1,Yang Linjun2,Xue Chunyan2 1 Department of Transfusion,Changhai Hospetal of Second Military Medical University(Shanghai 200433) 2 Department of Tumour,Changhai Hospetal of Second Military Medical University(Shanghai 200433);Biological Significance of Thrombocyte and Erythrocyte Adhesive Molecules CD_(35)、CD_(44) and CD_(62P) Express in Malignant Adults[J];Shenzhen Journal of Integrated Traditional Chinese and Western Medicine;2004-02
2 LIU Jing Han, OUYANG Xi Lin, Lü Liu Cai, Dayong Gao 1,2 (Department of Blood Transfusion, The General Hospital of PLA, Beijing 100853, China; 1Institute of Cryobiomedical Engineering, Faculty of Thermal Science and Energy Engineering, Chinese Science and Techinology University; 2University of Kentucky, Lexington KY40506 USA);Thermometry of Intracellular Ice Crystal Formation in Cryopreserved Platelets[J];Journal of Experimental Hematology;2002-06
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