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《Journal of Luzhou Medical College》 2010-01
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EFFECT OF STAT3 GENE SILENCING ON CELL CYCLE DISTRIBUTION AND APOPTOSIS LEVELS IN LUNG ADENOCARCINOMA A549 CELLS

Luo Linjie,et al Department of Respiratory Medicine,the First People's Hospital of Yibin City  
Objective:To investigate the changes of cell cycle distribution and apoptosis in lung adeno-carcinoma A549 cells before and after the STAT3 gene silencing and its significance. Methods:Before and after RNA interference (RNAi) technology silencing STAT3 gene expression in lung adenocarcinoma A549 cells ,the changes the cell cycle status and the level of apoptosis were detected by flow cytometry. Results:After STAT3 was silent,the lung adenocarcinoma A549 cells in the G0/G1 phase increased (P0.05),while in the S,G2/M phase decreased (P0.05); and apoptosis increased;in the first 48h ,the growth was significant (P0.05),after 48h ,the growth stabilized (P0.05),but still maintain a high level for a long time. Conclusion:STAT3siRNA can block the cell cycle of A549 cells and induce the apoptosis.It suggests that STAT3 expression in A549 cells are closely related with the cell cycle and apoptosis.
【Fund】: 四川省科技厅重点资助基金项目(2006J13-92)
【CateGory Index】: R734.2
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