Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Luzhou Medical College》 2010-05
Add to Favorite Get Latest Update

THE ESTABLISHMENT OF AN INTRACELLULAR FREE CA~(2+) CONCENTRATION MEASURING SYSTEM

Lei Ming,et al Institute of Myocardial Electrophysiology,Luzhou Medical College  
Objective:To establish an effective system for the measurement of intracellular free Ca2+ concen-tration.Method: Using Fura-2 as Ca2+ indicator,the fluorescence excited at 340nm and 380nm was detected un-der inverted fluorescence microscope,F340/F380 ratio was calculated which represents the relative Ca2+ concen-tration,and then standard Ca2+ concentration was calibrated.Result: F340/F380 steeply increased after stimulated with 10mM Caffeine,indicating the rapid increase of intracellular free Ca2+ concentration.This system responds quickly and sensitively to rapid changes of intracellular Ca2+ concentration.Conclusion: An effective system for the measurement of intracellular free Ca2+ concentration has been established and applied successfully in the mea-surement of intracellular free Ca2+ concentration of smooth muscle and myocardial cells.The first-hand data from this system will certainly be of value in the future.
【Fund】: 国家自然科学基金资助项目(No.30670763和No.30870903);; 四川省教育厅基金资助项目(No.2006ZD020)
【CateGory Index】: R337
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 LI Miao-Ling ZENG Xiao-Rong~* YANG Yan LIU Zhi-Fei DING Yin-Yuan ZHOU Wen PEI Jie Department of Myocardial Electrophysiology,Luzhou Medical College,Luzhou 646000,China;An improved method for isolation of single atrial myocyte from human heart[J];Acta Physiologica Sinica;2007-06
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 Li Tao,Li Miaoling,Lei Ming,Tan Xiaoqiu,Ding Yinyuan,Yang Yan,Zeng XiaorongDepartment of Electrophysiology,Institute of Cardiovasology,Luzhou Medical College;AN EFFECTIVE METHOD OF RECORDING SMALL CONDUCTANCE Ca~(2+)-ACTIVATED K~+ CHANNELS IN HUMAN ATRIAL MYOCYTE[J];Journal of Luzhou Medical College;2011-01
2 TONG Ting-hui,WANG Chun-yan , GUO Li. Department of Burns and Plastic Surgery , Affiliated Hospital of Luzhou Medical College , Luzhou 646000 , P. R. China;Influence of scald on the cytoskeleton of colonic smooth muscle cells of the rats[J];Chinese Journal of Burns;2006-04
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 ZHANG Biao~1, ZENG Xiao-rong~2, YANG Yan~2, LIU Zhi-fei~2, TONG Lin~3 (~1Department of Physiology, Guangdong Medical College, Zhanjiang 524023, China;~2Institute of Myocardial Electrophysiology, Luzhou Medical College, Luzhou 646000, China;~3The Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China);Acetylcholine sensitive K~+ channel (K_(ACh)) in chronic human atrial fibrillation[J];Chinese Journal of Pathophysiology;2006-04
2 ZHANG Yu, ZENG Xiao-rong, YANG Yan, ZHANG Biao, LIU Zhi-fei, LI Miao-ling, ZHOU Wen, PE1 Jie. Institute of Myocardium Electrophysiology, Luzhou Medical College, Luzhou 646000,Sichuan, China;Human inward rectifying potassium current and Kir2.1 mRNA expression in myocytes isolated from patients with chronic atrial fibrillation[J];Chinese Journal of Cardiology;2006-01
3 LI Miao-ling, ZENG Xiao-rong, YANG Yan, LIU Zhi-fei,ZHOU Wen,DING Yin-yuan, PEI Jie. Department of Myocardial Electrophysiology, Institute of Cardiovascular Research, Luzhou Medical College, Luzhou 646000, China;Research of L-type Ca~(2+) channel current in atrial myocytes of patients with chronic atrial fibrillation[J];Chinese Journal of Cardiology;2006-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved