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《Journal of Luzhou Medical College》 2013-01
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Comparative study of immunofluorescence staining of renal biopsy samples using frozen section and paraffin section

Zhang Cuiwei,Liu Yong,Zhang Xu,Ma Yuerong Department of Pathology,Luzhou Medical College  
Objective: To compare the effect of using frozen section and paraffin section on pathological diagnosis of renal biopsy samples by immunofluorescence staining.Methods: 40 cases of renal biopsy specimens were made of frozen section(control group) and paraffin section(experimental group).High-pressuer heatre was used in paraffin section group for antigen retrieval.Results of immunofluorescence staining were compared between the two groups.Results: The paraffin section group showed more false-negative results and non-specific staining.Compared with frozen section group,the fluorescence intensity of antibody IgG,IgA,C1q and C3 of paraffin section group were significantly different.Conclusion:The use of high-pressure heater for antigen retrieval in paraffin section may influence the pathological diagnosis of renal biopsy specimens.
【Fund】: 四川省科技厅项目(编号2010JY0080)
【CateGory Index】: R361.3
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