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《Scientia Silvae Sinicae》 2002-06
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IDENTIFICATION OF MICHELIA TSOI TYPES USING ISSR-PCR MARKER ASSAYS

Qiu Yingxiong Fu Chengxin(College of Life Sciences, Zhejiang University\ Hangzhou310029) He Yunfang(Management Station of Seed and Seedling,Zhejiang Forestry Bureau\ Hangzhou310029)  
Based on introduction and selective breeding of Michelia tsoi , genetic variations of 6 M. tsoi types were determined using ISSR marker assays. 12 primers were screened from 30 primers, and total 134 DNA bands were amplified, 67 of which (50%) were polymorphic. The average number of DNA bands amplified by each primer was 11.167. DNA profiles based on ISSR16, ISSR19 and ISSR2 have revealed potential diagnostic fingerprints and specific bands for various types. The ISSR specific bands are useful for the identification of cultivated germplasm, and testing trueness of cultivars. Key steps of ISSR\|PCR analysis techniques and application to testing trueness and purity of M. tsoi types were discussed. A dendrogram was constructed by using UPGMA algorithm.
【Fund】: 国家重点基础发展规划项目
【CateGory Index】: S792
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