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《Scientia Silvae Sinicae》 2008-09
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Fluctuating-Temperature Stratification Induced Seed Germination of Cistanche deserticola

Zhang Rumin~1 Bai Jing~2 Lü Chunling Chen Hongwei Gao Yan~1(1.School of Forestry and Biotechnology,Zhejiang Forestry University Lin'an 311300;2.Agronomy College,Inner Mongolia Agricultural University Hohhot 010019)  
In order to elucidate the mechanism of the seed germination in Cistanche deserticola,we investigated the effects of stratification treatment of high/low temperature on seed germination of C.deserticola using the tissue culture technique.The effects of high/low temperature stratification and plant hormones on seed germination rate of C.deserticola were investigated using the orthogonal experiment design.Results showed that the seed germination rate was 6.7% at 50 ℃ for 1 h and 1 week at(4 ℃) twice repeated stratification treatment for the seeds,and the germination took 60 d.To the contrast,the seed germination rate was 17.8% at 50 ℃ for 1 h and 1 week at 4 ℃ twice repeated stratification treatment combined with 1.4 μmol·L~(-1)6-BA,(2.9 μmol·L)~(-1) GA_3 and 5.8 μmol·L~(-1) IBA treatment,and the germination needed only 30 d.GA_3 was a key plant hormone to the seed germination to promote the germination rate at 50 ℃ and 1 w at 4 ℃.Two repeated stratification treatments and exogenous plant hormone were the essential conditions for the seed germination after being excised from host.
【Fund】: 国家自然科学基金资助项目(30360086);; 内蒙古自然科学基金资助项目(200208020502)
【CateGory Index】: S793
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