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《Scientia Silvae Sinicae》 2009-02
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Identification of Taxodium Hybrids by SRAP Analysis

Yu Chaoguang Yin Yunlong Xu Jianhua(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences Nanjing 210014)  
In this paper,the authenticity of 4 reciprocal progenies from Taxodium distichum and T.mucronatum was identified by SRAP(Sequence-related Amplified Polymorphism) markers.Firstly,48 SRAP primer combinations were randomly selected and polymorphism primers between two parents(T.distichum and T.mucronatum) were selected.As a result,all primers amplifid clear bands,and 29 primers had polymorphism bands of two parents,accounted for 60.4% of all primers,and 14 primer combinations with specific bands both two samples.Authenticity of 4 progenies from T.distichum and T.mucronatum was identified by 12 polymorphism primer combinations selected from above 29 polymorphism primer combinations.The results showed that 4 progenies were all identified to be true hybrids owing to their specific bands from male parent.Among the primer combinations,Me9+Em10 produced specific bands of both male parent and female parent in every progeny,that is to say,with this one primer combination alone,the status of 4 progenies can be identified.Efficiency of molecule identification were much improved.The results of hybrids identification provided a solid evidence for the hybrids further study and application.The experimental results also proved that SRAP molecular marker technology can be effectively applied in the researches on Taxodium.
【Fund】: 江苏省高技术(农业)研究项目(BG2007318);; 国家林业局植物新品种保护办公室植物新品种测试指南及已知品种数据库项目(2007010);; 江苏省林业三项工程项目(lysx200702)
【CateGory Index】: S791
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