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《Forest Research》 2008-06
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Analysis of Genetic Diversity in Lycoris radiata Using ISSR Marker

ZUO Hui1,2,YANG Zhi-ling1,YANG Xu1,TAN Zi-feng1,YU Hua-hui1 (1.Research Institute of Subtropical Forestry,CAF,Fuyang 311400,Zhejiang,China;2.Central South University of Forestry and Technology,Changsha 410004,Hu'nan,China)  
The genetic diversity of 14 Lycoris radiata populations was analyzed by ISSR markers.The results of POPGEN32 analysis showed that at species level,the percentage of polymorphics was 92.31%,Shannon's index(H) was 0.459 7,and Nei's gene diversity(I) was 0.302 5,indicating a high level of genetic diversity.But at population level,they were 49.65%,0.262 0 and 0.176 3,respectively,suggesting a low level of genetic diversity.And the gene differentiation coefficient(Gst) and gene flow(Nm) among the populations were 0.503 5 and 0.698 3,respectively.The analysis of molecular variance(AMOVA) demonstrated that there was a relatively high level genetic variation(46.12%) among the populations,and there was relatively low genetic variation(53.88%) within the populations.The high genetic differentiation among populations and the low genetic diversity within populations could be attributed to the habitat fragmentation and the limited gene flow among populations.
【Fund】: 国家科技部公益性林业专项项目“南方林源多用途药用植物种质保护和选育技术”(200704022);; 浙江省林业厅财政补助专项项目“林药(石蒜)生态经济型培育模式关键技术研究与示范”(07A03);; 浙江省重点农业项目“药用红花石蒜优质资源选育、野生驯化培育及离体培养再生体系的建立”(2004C22041);; 中国林科院亚热带林木培育实验室项目(特色药用植物专类园建设)部分内容
【CateGory Index】: S682.29
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