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《Journal of Liaoning University of Traditional Chinese Medicine》 2013-05
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Determination of Icariin Content in Energize Tablet by HPLC

XIONG Xianming,XIAO Yuqing,XIONG Hui,HE Mei,SONG Genwei(Renmin Hospital Affiliated to Hubei Medical University,Shiyan 442000,Hubei,China)  
Objective:To establish a rapid and simple method for the icariin determination of energize tablet.Method:High performance liquid chromatography was used.Column:SinoChromODS-BP C18(4.6 mm×250 mm,5 μm),mobile phase:acetonitrile:hydrogen oxide(29∶71),the detection wavelength:270 nanometer,fly velocity:1.0 mL per minute.Results:Icariin has a good linear relationship between 0.5 μg and 25.0 μg(r=0.9992,n=5).Conclusion:Experiments show that the method is simple,with good separation and reproducible,and can be used for detection icariin of energize tablet.
【Key Words】： energize tablet HPLC Icariin
【Fund】： 湖北省十堰市科学技术研究与开发项目(ZD2011022)
【CateGory Index】： R286
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