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《Journal of Liaoning University of Traditional Chinese Medicine》 2013-06
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Study on Determination of Geniposide and Chlorogenic Acid in Fructus Gardeniae

SHI Yuhong(The Fourth Hospital Affiliated to Liaoning University of Traditional Chinese Medicine,Shenyang 110101,Liaoning,China)  
Objective :To establish a method for determination of Geniposide and chlorogenic acid contents in Fructus gardeniae.Methods :Chromatographic column of Diamonsil C18 column(250 mm × 4.6 mm,5 μ m)was selected;the mobile phase consisted of 1% acetic acid aqueous solution-1.0% acetic acid acetonitrile gradient elution;the detection wavelength was 238 nm;the flow rate was 1 mL/min;column temperature was 30 ℃;sample was 10 μ L.Results :Jasminoidin in the concentration range of 3.171~126.85 μg/mL had good linear relationship,r=0.999(9 n=6),the average recovery was 99.98% and RSD was 0.33%;chlorogenic acid in the concentration range of 1.952~78.08 μg/mL had good linear relationship,r=0.9999(n=6),the average recovery was 100.6% and RSD was 0.81%.Conclusion :The method is simple,accurate and can be used for determination of geniposide,chlorogenic acid contents in Fructus gardeniae.
【CateGory Index】: R284
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