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《Bulletin of Botanical Research》 2007-06
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The Influence on Peroxidase Activity and Polyamine Oxidase Activity of Leaves in Wheat Seedings Exposed to Enhanced Ultraviolet-B Radiation and Irradiated by He-Ne Laser

MA Xiao-Li1,2 HAO Jin-Hua1 HAN Rong1*(1.School of Life Science,Shanxi Normal University,Linfen 041004)(2.Jinzhong College,Yuci 030600)  
The seedlings of wheat were exposed to He-Ne laser irradiation after enhanced UV-B radiated,in order to determine some physiological traits in the damage induced by UV-B radiation.The seeds were cultivated in Petridishes in a constant temperature incubator(25℃)until they were germinated.The germinated seeds were exposed to 10.08kJ·m-2·d-1 UV-B radiation,one by one,and then they were irradiated by He-Ne laser with 5mW·mm-2 Power density.Changes about the content of free proline(Pro) and the activity of the peroxidase(POD) and polyamine oxidase(PAO)were measured to test the repair role of laser irradiation.The results showed that the damage of wheat seedlings induced by enhanced UV-B radiation in physiology can be repaired partly by He-Ne laser irradiation with decreasing the content of free Pro and raising the activity of POD and PAO.This is the first investigation reporting the use of laser irradiation treatment to repair the wheat damage induced by UV-B radiation in peroxidase and polyamine oxidaset.It implied that the changes of PAO activity have the relativity with POD in some extent.
【Fund】: 国家自然基金资助项目 增强UV-B辐射与He-Ne激光对小麦细胞分束分裂影响机制的研究(编号:30671061);; 山西省自然科学资助项目(编号:20041101)
【CateGory Index】: Q945.7
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