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《药用植物研究(英文版)》 2010-09
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Determination of taxol content in the root of Taxus media at different ages by HPLC

CAI Yu-mei,LI Qing-lan,LIN Mao-fan,PU Shang-rao* Dujiangyan College of Sichuan Agricultural University,Dujiangyan,Sichuan 611830,China  
[Objective]To develop a reliable HPLC method for determining the dynamic change of taxol content in the root of Taxus media at different ages.[Method]The HPLC conditions were as follows:Phenomenex Luna C18 column(250 mm×4.6 mm,5 μm),mobile phase of methanol-acetonitrile-water,flow rate of 1.0 ml/min,detection wavelength of 227 nm,column temperature of 40 ℃ and injecting amount of 10 μl.[Result]The taxol was well separated under the above conditions.The linear relationship was good in the range of 1.00-32.00 mg/L(r=0.999 9),the average recovery rate and RSD values were 100.3% and 1.6% respectively.The content of taxol in the root of Taxus media at different ages was varied.[Conclusion]A reliable and convenient method was firstly established to determine the taxol content in the root of Taxus media.
【Fund】: Supported by Key Project of Sichuan Provincial Education Department(2003A023)
【CateGory Index】: O657.72;R284
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