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《药用植物研究(英文版)》 2012-02
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Effect of Different Extraction Methods on the Chemical Constituents of Essential Oils from FOLIUM ARTEMISIAE ARGYI

SHI Lin*,YANG Yuan-e,YAO Yong-fang Department of Food and Bioengineering,Guangdong Industry Technical College,Guangzhou,Guangdong 510300,China  
[Objective] To study the effect of different extraction methods on the chemical constituents of essential oils from FOLIUM ARTEMISIAE ARGYI.[Method] The essential oils were first extracted from FOLIUM ARTEMISIAE ARGYI by steam distillation and SFE-CO2,and then qualitatively identified by GC-MS.Next,the relative content of each component was calculated by normalization method.[Result] There were significant differences in the chemical constituents of essential oils that were extracted from FOLIUM ARTEMISIAE ARGYI by different methods like steam distillation and SFE-CO2.The essential oils extracted by SFE-CO2 mainly contained 1,2-benzenedicarboxylic acid-2-ethylhexyl mono ester(17.81%) and 1,2,3,4,4a,5,6,8a-octahydro-7-methyl-4-methylene-1-(1-methylethyl)-(1a.alpha.,4a.alpha.,8a.alpha.)-naphthalene(11.13%),while that extracted by steam distillation mainly contained 4,6,6-trimethyl-[1S-(1a,2β,5a)]-bicyclo [3.1.1] hept-3-en-2-ol(22.05%),4-methyl-1-(1-methylethyl)-[1S-(1α,4β,5α)]-bicyclo [3.1.0] hexan-3-one(10.69%) and caryophyllene oxide(10.62%).[Conclusion] The essential oils extracted from FOLIUM ARTEMISIAE ARGYI by SFE-CO2 contained more chemical constituents,especially low-polarity substance.However,the chemical constituents of essential oils extracted by steam distillation focused on higher-polarity alcohols.It was difficult to extract low-polarity volatile components by steam distillation.
【Fund】: Supported by the Initial Foundation for Scientific Research of Guangdong Industry Technical College (KXKY200904)
【CateGory Index】: R284
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