Study on the screening of strains with high activity acid protease for wool
CHEN De-zhao, ZHANG Tong-liang, ZHANG Yang (College of Chemistry and Chemical Engineering of Donghua University,Shanghai 200051,China)
A high yield acid protease producing strain Asp.niger DH-C40 is screened from its parent strain Asp.niger DH-010 by treating with UV and nitrous acid. The acid protease is good at decompose wool. Invent a new way to determine the activity of the enzyme for wool. Define 1 ml enzyme decompose 1 μg wool in one hour as 1 unit activity of enzyme. A new screening culture medium was designed. Using the new culture medium, 7 strains which can produce the enzyme for wool are screened. Using the flask bottle to screen among the 7 strains, we get a high yield strain——DH-C40. Using the new determine way, DH-40 can produce a high yield acid protease for wool, the activity of protease is 620 μg/ml.
【CateGory Index】： TS101.4