Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
Add to Favorite Get Latest Update

Somatic Embryogenesis and Plant Regeneration of Chuan239 of Upland Cotton

GUO Yu long 1, LI Ming yang 1, PEI Yan 1, CAI Ying fan 2 (1 Center of Biotechnology, Southwest Agricultural University chongqing 400716, China; 2 Cotton Research Institute, Sichuan Academy of Agricultural Sciences 641400, China)  
The Chuan 239 planted in the Yangtze River Cotton Region is a recalcitrant cotton variety for somatic embryogenesis. To achieve somatic embryogenesis from it, several kinds of hormone and subculture methods were investigated. The medium MSB containing 2,4 D 0 05mg·L -1 +KT0.1mg·L -1 or NAA 4mg·L -1 +KT1mg·L -1 was good for callus initiation but the callus didn't produce embryos after subculture. The medium MSB Containing IAA0 5mg·L -1 +KT0 1mg·L -1 or ZT0 1mg·L -1 only yielded a very low inducing frequency and growth speed of callus, but all the callus were embryogenic. When the embryogenic callus were subcultured with SM 1(MSB+IAA0 1mg·L -1 +KT0 1mg·L -1 ) and SM 2(MSB+KT0 1mg·L -1 +KNO 3 1 9g·L -1 ) alternatively, the growth of nonembryogonic callus were retarded and somatic embryos were produced. Suspension culture promoted somatic embryogenesis. For germination of somatic embryos, the medium SH with 0 5g·L -1 active coal was better than that of medium MSB with BA 0 1mg·L -1 and IAA0 1mg·L -1 or hormone free MSB medium. Regenerated plants from somatic embryos were obtained. The strategy to achieve somatic emdbryogenesis from recalcitrant cotton varieties was discussed.
【Fund】: 中华农业科教基金;四川省生物技术项目
【CateGory Index】: S562.035
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 Qin Yonghua Liu Jinyuan* Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084;Cotton Tissue Culture and Plant Regeneration[J];Molecular Plant Breeding;2006-04
2 GUO Yu-Long1, 2 LU Xiu-Min1* ZHU Qin-Long2 LI Ming-Yang2 PEI Yan1 (1.Center of Biotechnology, Southwest Agricultural University, Chongqing 400716, China; 2. Horticulture Cellege, Southwest Agricultual University, Chongqing 400716, China);Genetic Transformation of Cotton Embryogenic Callus by Particle Bombardment and Regeneration of Transgenic Plants[J];Journal of Agricultural Biotechnology;2005-02
3 XUE Meifeng,GUO Yulong,LI Mingyang,PEI Yan(Center of Biotechnology,Southwest Agricultureal University,Chongqing 400716,China);Effects of long-term subculture on embryogenesis capability of cotton callus and somatic variation[J];Southwest China Journal of Agricultural Sciences;2002-04
Chinese Journal Full-text Database 2 Hits
1 Zhang Xianlong Zhang Jiaming Yiao Mingjing Sun Jizhong Liu Jinlan (Huazhong Agricultural University, Wuhan 430070);STUDIES ON SOMATIC CELL CULTURE AND ITS APPLICATION IN COTTON (GOSSYPIUM L. )[J];Journal of Huazhong Agricultural;1993-05
2 Chen Zhixian Li Shujun (Cotton Res.Ins., Shanxi Acad. of Agri. Sci., Yucheng) N.L.Trolinder (Cropping Systems Res. Lab., USDA-ARS, Lubbock, Texas 79409) J.R.Goodin (Texas Tech. University, Lubbock, Texas 79409);SOME CHARACTERISTICS OF SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN UOTTON CELL SUSPENSION CULTURE[J];Scientia Agricultura Sinica;1987-05
Chinese Journal Full-text Database 10 Hits
1 Wu Fuan Song Qingqi He Zengyong Zong Qiuling (Shandong Cotton Research Centre);SCREENING OF LINES WITH HIGH FREQUENCE OF CELL REGENERATION FROM ELITE NOVEL STRAINS OF UPLAND COTTON(G. HIRSUTUM L.)[J];Shandong Agricultural Sciences;1991-05
2 LI Zhanqi Liu Dajun(Southwest China Normal University,Chongqing 400715,China);Effects of Different Cultural Conditions on culturing of Xiangmian 18 cotton Stem Tip Tissue[J];Anhui Agricultural Science Bulletin;2007-16
3 XUE Yuanchao Liu Dajun(College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China);Initill study on Tip Tissue Cultural Condition of Xiangmian 18 Cotton[J];Anhui Agricultural Science Bulletin;2007-16
4 Zhang Baohong(COtton Research Institute,Chinese Academy of Agricultural Sciences)Guo Denglong(Hezhe Institute of Agricultrual Science);Achievement of Studies on Cotton Biotechnology and Its Developmental Strategy in China[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;1995-02
5 Zhang Baohong;Li Fuguang;Wang Wu;Li Fenglian;Li Xiulan(Cotton Research Institute , Chinese Academy of Agricultural Sciences,Anyang455112 ,Henan);Somatic Embryogenesis and Plant Regeneration of An Elite Cotton Variety, Simian No.3[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;1995-03
6 Zhang HanshuangLi JunlanGao PengWang Haibo (Cotton Insititute,HAAFS, Shijiazhuang 050051);Embryogenesis and Plant Regeneration from SomaticCells Culture of Glandless Upland Cotton[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1999-01
7 Chi Jina Ma Zhiying* Zhang GuiyinKey Laboratory for Germplasm Resources of Hebei Province, Agricultural University of Hebei, Baoding, 071001*Corresponding author, mzhy@mail.hebau.edu.cn;Genotypes Documentation of Cotton in Somatic Cells Plant Regeneration in China[J];Molecular Plant Breeding;2005-01
8 He Yating 1 Qu Yanying 1 Kong Qingping 2 Yan Yongliang 1 Shang Shuang 1 Zheng Liangyu 1 Chen Quanjia 11 Agriculture College of Xinjiang Agriculture University, Urumqi, 830052; 2 Institute of Cash Crops Research, Xinjiang Academy of Agricultural Sciences, Urumqi, 830000;Primary Studies on the Effects of Callus Induction and Differentiation in Gossypium Barbadense[J];Molecular Plant Breeding;2008-03
9 Dong Hezhong Jiao Gaili Chen Zhixian(Cotlon Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000);The Effect of 2,4-D and KT on Callus Initiation and Somatic Embryogenesis in Cotton Culture[J];Acta Agriculturae Boreali—Sinica;1993-03
10 LI Jun lan,ZHANG Han shuang,GAO Peng,WANG Hai bo (Cotton Research Institute,Hebei Academy of Agricultural and Forestry Science,Shijiazhuang 050051,China);Study on the Transformation of Cotton Mediated with Agrobacterium tumefacines[J];Acta Agriculturae Boreall—sinica;2002-S1
Chinese Journal Full-text Database 10 Hits
1 Wu Fuan Song Qingqi He Zengyong Zong Qiuling (Shandong Cotton Research Centre);SCREENING OF LINES WITH HIGH FREQUENCE OF CELL REGENERATION FROM ELITE NOVEL STRAINS OF UPLAND COTTON(G. HIRSUTUM L.)[J];Shandong Agricultural Sciences;1991-05
2 Li Shuying et al (Cotton Institute, AAAS, Anqing 246003);Advanced Researches on the Mechanism of Virulence of Verticillium dahliae and Resistance Breeding in Cotton[J];Journal of Anhui Agricultural Sciences;2001-04
3 Wang Xingfen;Zhang Guiyin;Li Ruiqi;Ma Zhiying (Shool of Agronomy, Hebei Agtic Univ, Baoding 071001);Inheritance of Resistance to Verticillium wilt in Gossypium hirsutum[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1999-02
4 Zhang HanshuangLi JunlanGao PengWang Haibo (Cotton Insititute,HAAFS, Shijiazhuang 050051);Embryogenesis and Plant Regeneration from SomaticCells Culture of Glandless Upland Cotton[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1999-01
5 Li Keqin;Wang Zhezhi;Zhang Dali;Guo Dezhi;HaoLianfang;Zhang Sufeng;Sheng Zhigang, (Department of Biology, Shaanxi Normal University, Xi'an 710062);STUDIES ON TISSUE AND CELL CULTURE OF GOSSYPIUM HIRSUTUM L.[J];Acta Botanica Boreali-occidentalia Sinica;1991-02
6 YU Ya~1,LIU Chuan-liang~2 ,MA Zhi-ying~1,LI Fu-guang~(2*), LI Feng-lian~2,WANG Yu-fen~2,WU Zhi-xia~2,ZHANG Chao-jun~2(1 College of Agriculture, Agriculture University of Hebei ,Baoding, Hebei 071001,China;2 Chinese Cotton Research Institute,Chinese Agricultural Academy of Sciences,Anyang Henan 455000,China);Embryogenic callus induction and plant regeneration from "CCRI 24[J];Acta Botanica Boreali-occidentalia Sinica;2004-02
7 Zhang Baohong\ Feng Rong\ Du Xongming\ Li Fenglian Wang Chunying\ Feng Xinai\ Li Xiulan (Cotton Research Institute,Chinese Academy of Agricultural Sciences,Anyang Henan 455112);Scanning Electron Microscope Observation on Embryogenesis and Organogenesis in Tissue Culture of Gossypium klotzschanium Anderss[J];JOURNAL OF CHINESE ELECTRON MICROSCOPY SOCIETY;1997-02
8 LUO Qi, WANG Jianhua, WANG Yuanliang, PAN Junmechanics and Tissue Engineering under the state Ministry of Education, Chongqing 400044,China);Regulation and Control of Metal ion in Cell Apoptosis[J];Journal of Chongqing University(Natural Science Edition);2003-08
9 Wang Lihua Dai Xiaofeng * Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081;Progress on Molecular Research of Cotton Verticillium wilt Resistances[J];Molecular Plant Breeding;2003-01
10 Postgraduate: Mr. Kong W. Supervisor: Dr.& Prof. Fang X.J. Postgraduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081;Cloning and Mapping of NBS-LRR Type Resistance Gene Analogs in Cotton (Gossypium barbadense L.)[J];Molecular Plant Breeding;2003-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 YANG Bing-bing,XIA Han-ping,MA Zhen-rong (South China Botanical Garden,the Chinese Academy of Sciences,Guangzhou 510650,China);Study on tissue culture of Vetiveria zizanioides[J];Acta Prataculturae Sinica;2007-04
2 CHEN Fa-ju1,ZHAO Zhi-gang1,LIANG Hong-wei1,XIONG Dan1,LI Feng-lan2(1 Biotechnology Research Center,and Hubei Province Key Laboratory of Natural Products Research and Development,China Three Gorges University,Yichang,Hubei 443002,China;2 School of Biological Science and Biotechnology,Beijing Forestry University,Beijing 100083,China);Chromosome Number Variation of Cells in Reiterative Subcultures of Embryonic Callus of Tapiscia sinensis Oliv.[J];Acta Botanica Boreali-Occidentalia Sinica;2007-08
3 MA Hui-ling,LI Yu-zhu(College of Prataculture,Gansu Agricultural University,Lanzhou 730070,China);Analysis of influence factors on plant protoplast culture[J];Journal of Gansu Agricultural University;2007-04
4 FENG Da-ling1,MENG Qing-rong2,LI Wen-ping2,HU Yong-hong2,LI Ming1,GU Ai-xia3 1 College of Life Science,Agricultural University of Hebei,Baoding 071001,P.R.China 2 Baoding Research Institute of Agricultural Science,Baoding 071001,P.R.China 3 College of Landscape Architecture and Tourism,Agricultural University of Hebei,Baoding 071001,P.R.China;Morphology of somatic embryogenesis and plantlet formation in tissue cultures of lantern tree (Koelreuteria bipinnata var.integrifoliola)[J];中国林学(英文版);2010-01
5 SUN Jing-yan1,LI Wei-ming1,ZHANG Han-shuang1,ZHAO Jun-li1,WANG Yong-qiang1,WANG Li-an2(1.Institute of Cotton,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,China;2.College of Life Science,Hebei Normal University,Shijiazhuang 050016,China);Establishment of Agrobacterium-mediated Transformation System for Embryogenic Callus and Acquirement of Transgenic Plants with DREB1C Gene in Glandless Cotton[J];Journal of Hebei Agricultural Sciences;2009-06
6 Fu Yuanzhi,et al.(Henan Institute of Science and Technology,Xinxiang 453003,China);Effect of Several Different Facts on Callus induction of Baimian N0.1[J];Journal of Henan Institute of Science and Technology(Natural Sciences Edition);2008-03
7 ZHOU Zhi-lin1,2,NIE Yi-chun1,ZHANG Xian-long1,HU Ting-ting2(1.National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China;2.Xuzhou Institute of Agricultural Sciences of the Xuhuai District,Xuzhou 221121,China);Chromosomal Variations during Culture of Somatic Cells of Cotton[J];Jiangsu Journal of Agricultural Sciences;2008-02
8 LI Fu-guang,LIU Chuan-liang(Cotton Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Anyang,Henan 455000,China);Applications of Biotechnology on the Cotton Breeding[J];Cotton Science;2007-05
9 Meng Si,*Liu Xiaoyu(College of Food Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China);Advances of Research on Cotton Transgene Technology and Its Target Characters[J];Academic Periodical of Farm Products Processing;2009-05
10 WEI Yan-li1,HUANG Yu-jie1,LI Hong-mei1,SUN Hong-xing1,2,YANG He-tong1,2 (1.Biotechnology Research Center,Shandong Academy of Sciences;Key Laboratory of Applied Microbiology of Shandong Province,Jinan 250014,China;2.School of Life Sciences,Shandong University of Technology,Zibo 255049,China);A Survey of Cotton Transgene Technology[J];Shandong Science;2008-03
China Proceedings of conference Full-text Database 1 Hits
1 FU Yuan-zhi WANG Qing-lian LU Yu-zhen HU Gen-hai (Henan Institute of Science and Technology,Xinxing,henan 453003);Effect of Several Different Facts on Callus induction of Baimian NO.1[A];[C];2007
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 ZHANG Jia-ming LIU Jin-lan SUN Ji-zhong(Huazhong Agricultural University, Wuhan 430070);GERMINATIOM OF SOMATIC EMBRYOS AND PRELIMINARY STUDIES ON VARIATION OF REGENERATED PLANTS IN UPLAND COTTON[J];Journal of Huazhong Agricultural;1992-01
2 Zhang Xianlong Yao Mingjing Liu Jinlan Sun Jizhong (Huazhong Agricultural University, Wuhan 430070);EFFECTS OF TOXIC FILTRATES PRODUCED BY FUSARIUM WILT DISEASE STRAIN ON SEED GERMINATION AND TISSUE GROWTH OF COTTON[J];Journal of Huazhong Agricultural;1993-04
3 Zhang Xianlong Sun Jizhong Liu Jinlan (Department of Agronomy,Huazhong Agricultural University,Wuhan);A Comparative Study on the Biochemical Metabolites between the Embryogenic and non-embryogenic Calli from the Variety“Coker 201”of Gossypium hirsutum L.[J];Acta Agronomica Sinica;1992-03
4 Zhang Xianlong Sun Jizhong Liu Jinlan(Huazhong Agricultural University, Wuhan 430070);Somatic Embryogenesis and Plant Regeneration in Upland Cotton[J];Acta Genetica Sinica;1991-05
5 WANG Wu;ZHANG Xian-Long;LIU Jin-Lan Department of Agronomy, Central-China Agricultural University, Wuhan 430070;Effects of Brassinolide on Somatic Embryogenesis of Gossypium hirsutum[J];Plant Physiology Communications;1992-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved