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《Acta Gossypii Sinica》 2002-06
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Improvement of Plant Regeneration via Somatic Embryogenesisin Upland Cotton (Gossypium hirsutum L.)

CHEN Meiyou, ZHANG Xianlong*, NIE Yichun, WU Jiahecultural University, Wuhan 430070, China)  
s: Special effects of IBA on somatic embryogenesis and plant regeneration of upland cotton were investigated, and the system of plant regeneration has been improved. The medium MSB (MS inorganic salts and B5 vitamins) containing IBA 1.0mg·L1 and KT0.5mg·L1 can induce a number of embryogenic callus from Coker 201 in two months. And somatic embryos can be observed in the third month since inoculation. Meanwhile, adding an appropriate amount of AgNO3 could promote callus proliferation in subculture. IBA 0.5mg·L1 combining with KT0.1~0.2mg·L1 is very good for subculture and development of embryoid. Adding KNO3 1.9g·L1 and using mixture of sucrose 15g·L1 and glucose 15g·L1 as carbon sources can stimulate formation and germination of embryoids. The medium MS with 0.4g·L1 active charcoal was appropriate for embryoid to develop plantlets. For root induction, plantlets need to be transferred to a 1/6 MS liquid medium without mInositol. A number of plantlets have been obtained in six months since inoculation of explants.
【Fund】: 948"(201012);; 湖北省自然科学基金重点项目(2000J095)
【CateGory Index】: S562.035
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