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《Acta Gossypii Sinica》 2005-04
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Technical Improvement of Tissue Culture and Somatic Embryogenesis in Upland Cotton

CHI Ji-na, MA Zhi-ying , HAN Gai-ying, LI Xi-huan, WANG Yan-xia {Agricultural University of Hebei, Key Laboratory for Crop Germplasm Resources of Hebei Province, Baoding 071001, China)  
Two varieties of upland cotton, Coker 312 and Jiwu 2031, were studied on somatic embryo-genesis. They produced different amount of embryogenic calli and regenerated plants. A better procedure of cotton embryogenic callus inducement was established. Special effects of PEG6000 on somatic embryogenesis in upland cotton were investigated. PEG6000 could raise ratio of embryoids germination remarkablely, which got the highest rate of embryoids germination in the treatments of 50 g·L-1 in suspension culture. It promoted embryogenic callus germination in normal light period in two weeks after one or two weeks in darkness with 80-160 mg·L-1 of PEG6000 in solid medium. This method was also applied to maintenance embryonic callus. MSB(MS inorganic salts and B5 vitamin) with phy-tohormone free, 500 mg·L-1 of CH, half amount of NH4NO3 and double amount of KNO3 could stimulate formation and germination of embryoids, and reduce abnormal embryoids.
【Fund】: 河北省科技攻关项目(03220137D)
【CateGory Index】: S562
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