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《Acta Gossypii Sinica》 2005-05
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Prevent Browning of Upland Cotton Embryoid Callus

ZHANG Chao-jun,LI Fu-guang~*,WANG Yu-fen,WU Zhi-xia,LI Feng-lian(Cotton Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Anyang,He′nan 455112,China)  
In cotton tissue culture,normal green embryoid is usually obtained after 3 or 4 rounds subcultures.But the callus usually turns brown in the subculture from embryogenic callus to somatic embryogenesis.Our research suggests that the brown-turning mainly results from the improper ratio of nitrogen.It also indicated that the content of KT、6-BA and IAA had no significant effect on embryo tissue brown-turning.The normal green embryoid is produced only by once subculture after improvement of MS culture medium,shorten its cycle about two months.
【Fund】: 国家“863”转基因棉育种技术(2004AA212100)
【CateGory Index】: S562
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