Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Cotton Science》 2008-02
Add to Favorite Get Latest Update

Molecular Cloning and Expression Analysis of Two Chitinase in Upland Cotton

YANG Yu-wen, ZHANG Bao-long, NI Wan-chao, SHEN Xin-lian, ZHANG Xiang-gui, XU Ying-jun(Institute of Agriculture Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)  
ESTs encoding class Ⅲ and classⅣ chitinase respectively were isolated from the SSH library inoculated with Verticillium wilt. Both of them contained full 3’ end and their full open reading frame were obtained by RACE and RT-PCR. As a result, the entire coding region of class Ⅲ and classⅣ chitinase is 1390 bp and 678 bp respectively and encodes a polypeptide of 298 and 226 amino acids. The polypeptide encoded by these two genes shared 71% and 65% homology to their homologous proteins of Arabidopsis. They were designated as Ghachi3 and Ghachi4. All of these two genes have signal peptide and were predicted to be extracellular chitinase. Ghachi3 were abundant in petals, buds and phloem, whereas Ghachi4 only showed an extremely high level in phloem by semi-quantitative RT-PCR. The accumulation of Ghachi4 increased remarkably after treatment of Verticillium wilt and Fusarium wilt in the disease resistant cultivar exclusively. But Ghachi3 was also induced by Verticillium wilt in the susceptible cultivar. In addition, the expression of both the two genes increased remarkably after treatment of ABA. All the results indicated that these two genes might play roles in response to biotic and abiotic stresses.
【Fund】: 江苏省农业科学院科研基金(6110528)
【CateGory Index】: S562
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 4 Hits
1 ZHANG Chun-ying,WANG Xing-fen,ZHANG Gui-yin,WU Li-qiang,CHI Ji-na,LI Zhi-kun,MA Zhi-ying (Key Laboratory of Crop Germplasm Resources of Hebei/ Agricultural University of Hebei,Baoding,Hebei 071001,China);ESTs Analysis of Suppression Subtractive Hybridization Library from Upland Cotton Resistant Cultivar Infected by Verticillium dahliae[J];Cotton Science;2010-01
2 YANG Yu-wen,HE Bing,ZHANG Bao-long,NI Wan-chao (Institute of Agribiotechnology,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Jiangsu Agricultural Biology,Nanjing 210014,China);Molecular Cloning and Expression Analysis of a Receptor-like Protein Kinase Gene in Upland Cotton[J];Cotton Science;2011-01
3 LIU Dong-feng, ZHANG Chao-hong, WANG Yue-jin (Key Laboratory of Horticultural Plant Germplasm Resource Utilization in Northwest China, Ministry of Agriculture; Key Laboratory of Molecular Biology of Agriculture, Shaanxi Province; College of Horticulture, Northwest Agriculture and Forestry University,Yangling 712100,China);Full-length cDNA Clone and Sequence Analysis of the Chitinase Family Gene from Pear[J];Journal of Agricultural Biotechnology;2009-06
4 MA Yin-Ping1,2,WANG Fu-Xin2,YANG Chun-Lin2,SHEN Fa-Fu1,XIA Gui-Xian2 1.State Key Laboratory of Crop Biology,College of Agronomy,Shandong Agricultural University,Tai'an 271018,China; 2.State Key Laboratory of Plant Genomics,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;Cloning and functional analysis of chitinase gene GbCHI from sea-island cotton(Gossypium barbadense)[J];Hereditas;2012-02
Chinese Journal Full-text Database 9 Hits
1 ZHANG Zhi-zhong,WU Jing-hua,Lü Liu-xin,LIN Yi-zhang(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Progress of plant chitinase and its application[J];;2005-04
2 LIN Ling~1,ZHAN GAi-xiang~1,JIN Zhong-shi~2,WANG Yong-shan~2,WANG Feng-liang~2,GONG Wei-rong~3,CHEN Zhi-shi~1,GU Ben-kang~1 (1.Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2.Dafeng Plant Protection and Quarantine Station, Dafeng 224100,China;3.Jiangsu Plant Protection Unit,Nanjing 210013,China);Effects of Bio-control Bacterium and Fulvic Acid on Cotton Growth and Verticillium Wilt[J];Jiangsu Journal of Agricultural Sciences;2006-02
3 ZHOU Miaoping, LU Weizhong (Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing210014);Plant Chitinases and Its Application in Anti fungal Gene Engineering[J];JIANGSU JOURNAL OF AGRICULTURAL SCIENCES;1998-03
4 ZHANG Zhi-gang1,2,3,QIU De-wen2*,YANG Xiu-fen2,Zeng Hong-mei2,YUAN Jing-jing2,LIU Zheng2,YANG Xiao-ping3,GUAN Chun-yun2(1.Hunan Agriculture University,Changsha 410128,China;2.Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3.Hunan Cotton Research Institute,Changde 415000,China);Construction of Suppression Subtractive Hybridization Library with cDNA from Seedling Cotton Induced by Protein Elicitor from Alternaria tenuissima and Analysis of EST[J];Cotton Science;2007-04
5 WANG Xue,MA Jun,ZHANG Gui-yin,LI Xi-huan,WANG Xing-fen*,MA Zhi-ying*(Agricultural University of Hebei,Key Laboratory of Crop Germplasm Resources of Hebei,Baoding 071001,China);Proteomic Analysis of Cotton Leaf under Verticillium dahliae Stress[J];Cotton Science;2007-04
6 LI Fu-guang,LIU Chuan-liang(Cotton Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Anyang,Henan 455000,China);Applications of Biotechnology on the Cotton Breeding[J];Cotton Science;2007-05
7 WU Jia-He~(1,2),ZHANG Xian-Long~(1,①),LUO Xiao-Li~2,NIE Yi-Chun~1,TIAN Ying-Chuan~3,CHEN Zheng-Hua~4 (1.National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan430070,China; 2.Cotton Research Institute,Shanxi Agricultural Academy,Yuncheng044000,China; 3.Institute of Microbiology,Chinese Academy of Science,Beijing100080,China; 4.Institute of Genetics and Developmental Biology,the Chinese Academy of Sciences,Beijing100101,China);Transgenic Cotton Plants of Chitinase and Glucanase Genes and Their Performance of Resistance to Verticillium dahliea[J];Acta Genetica Sinica;2004-02
8 Cai Xinzhong Zheng Zhong (Department of Plant Protection,Zhejiang University, Hangzhou 310029);MECHANISMS AND PATHWAYS OF PLANT SYSTEMIC ACQUIRED RESISTANCE[J];Journal of Plant Protection;1999-01
9 LUO Ping, WANG Guo_Dong, CHEN Xiao_Ya * [WT6BZ](National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, Shanghai 200032, China);Isolation and Expression Analysis of Two cDNAs Encoding C4H Homologues from Gossypium arboreum[J];Acta Botanica Sinica;2001-01
Chinese Journal Full-text Database 10 Hits
1 Anhui Agricultural University, Hefei 230036 );Plant Elicitors of BenzothiadiazoleChu Mingjie Huang Dezhi[J];Anhui Chemical Industry;2003-02
2 ZHONG Wei-ran et al (College of Agronomy and Biotechnology,Southwest University,Chongqing 400716);Study on the Cytochrome P450s in Phenylpropanoid Metabolic Pathway[J];Journal of Anhui Agricultural Sciences;2008-13
3 YANG Li-heng et al (College of Agronomy,Xinjiang Agricultural University,Urumuqi,Xinjiang 830052);Study on the Transformation Method of Mediating Exogenous Gene into Cotton Epicotyl by Agrobacterium[J];Journal of Anhui Agricultural Sciences;2008-19
4 JIN Hua et al(College of Life Science,Dalian Nationalities University,Dalian,Liaoning 116600);Molecular Cloning and Sequence Analysis of Class Ⅱ Chitinase Gene in Leymus chinensis[J];Journal of Anhui Agricultural Sciences;2009-31
5 SUN Hui et al(Department of Plant Protection,Zhongkai University of Agriculture and Engineering,Guangdong,Guangzhou 510225);Application of Subtractive cDNA Library in Study of Plant Resistance[J];Journal of Anhui Agricultural Sciences;2010-05
6 GAN De-fang1,WANG Xiao-jun2(1.School of Horticulture,Anhui Agricultural University,Hefei 230036;2.Agency of Shanghua Street of Lanxi City in Zhejiang Province,Lanxi 321100);Study on methods of extracting total RNA from Chinese cabbage leaves[J];Journal of Anhui Agricultural University;2007-02
7 WANG Wan-neng,QUAN Xue-jun, ZHOU Yue-gang,HU Yong (College of Bioengineering, Chongqing Institute of Technology, Chongqing400050,China);Research on the Biological Control of Plant Diseases[J];Journal of Chongqing Institute of Technology;2004-02
8 HAN Dang-yue1,DU Shao-hua1,XU Yang1,SONG Yan-xiang1, LI Zheng-nan3,RAN Long-xian1,2(1.College of Forestry,Agricultural University of Hebei,Baoding 071001,China; 2.Hebei Key Lab of Forest Germplasm Resources and Protection,Agricultural University of Hebei, Baoding 071001,China;3.Mountain Area Research Institute of Hebei Province,Baoding 071001,China);Induction of systemic resistance against jujube fruit shrinking disease with salicylic acid[J];Journal of Agricultural University of Hebei;2012-03
9 JIA Xiao-xia1,ZHANG Jin-wen1,WANG Han-ning1,MA Huai-yi1,LIANG Hui-guang2(1.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China;2.College of Science,Gansu Agricultural University,Lanzhou 730070,China);Construction of plant expression vectors containing two anti-fungal and one anti-herbicide genes and their expression in transgenic tobacco[J];Acta Prataculturae Sinica;2009-01
10 LI Zhen-dong1,CHEN Xiu-rong1,LI Peng1,MAN Bai-ying2(1.Pratacultural College,Gansu Agricultural University,Key Laboratory of Grassland Ecosystem Ministry of Education,Sino-U.S.Centers for Grazingland Ecosystem Sustainability,Agricultural University,Lanzhou 730070,China;2.Guangzhou University Sontan College,Guangzhou 511370,China);Identification of Polygonum viviparum endophytic bacteria Z5 and determination of the capacity to secrete IAA and antagonistic capacity towards pathogenic fungi[J];Acta Prataculturae Sinica;2010-02
China Proceedings of conference Full-text Database 4 Hits
1 HUANG Li-zhi,ZHANG Chun-ying,ZHANG Yan,LI Zhi-kun,WU Li-qiang,WANG Xing-fen,ZHANG Gui-yin,MA Zhi-ying(North China Key Laboratory for Crop Germplasm Resources of Education Ministry/Key Laboratory for Crop Germplasm Resources of Hebei/Agricultural University of Hebei,Baoding,Hebei 071001,China);Cotton(Gossypium hirsutum) Cyclophilin Purification and Antagonistic Activity Assay to Verticillium dahliae[A];[C];2012
2 Jian Guiliang,Zou Yafei,Iiu Huijun (Institute of Plant Protection,CAAS,Beijing 100094);Trans Chi Gene to Insect Resistant Cotton Influence on Agricutural Features[A];[C];2003
3 TONG Yunhui, GUO Guiping, XU Jingyou, Ji Zhaolin, CHEN Xijun (1. Department of Plant Protection, Agricultural College, Yangzhou University, Yangzhou 225009,China; 2. Nantong Entry-Exit Inspection and Quarantine Bureau, Nantong 226005, China);INDUCED RESISTANCE TO GRAY MOULD IN TOMATO PLANT BY ANTAGONISTIC BACTERIA[A];[C];2003
4 LIU Tai-guo,LI Yong-hao,CHEN Wan-quan Institute of Plant Protection,Chinese Academy of Agriculture Science,Beijing 100094,China; Department of Plant Protection,Northeast Agricultural University,Harbin,Heilongjiang 150030,China;Effects on the PAL activity in tobacco leaves after treated with salicylic acid and inoculated with tobacco mosaic virus[A];[C];2005
Chinese Journal Full-text Database 10 Hits
1 Ma Wenyue (Department of Agronmy, Guizhou University, Guiyang,Guizhou 550025);Studies on Chilling Injury and Cold Hardiness of Plants[J];Journal of Anhui Agricultural Sciences;2004-05
2 QIU Xue-si et al (Department of Horticulture,Shenyang Agricultural University, Shenyang,Liaoning 110161);Dynamic Changes of Endogenous hormonein Leaf During the Period of Flower Bud Differentiation of Apricot[J];Journal of Anhui Agricultural Sciences;2006-09
3 LIU Xiao-lin(College of Life Science and Resource Environment,Yichun University,Yichun,Jiangxi 336000);Proteomics and Its Research Progress[J];Journal of Anhui Agricultural Sciences;2007-31
4 WU Jun-chao, JIANG Ying (Biochemistry Department of Medical College of Suzhou University, Suzhou 215007);Development of Rapid Amplification of cDNA Ends[J];Amino Acids & Biotic Resources;2003-01
5 Li Xinghong; Ma Zhiying; Zhang Guiyin(Hebei Agricultural University, Baoding 071001);The Advance of Study on Mechanism of Verticillium Wilt Resistance in Cotton[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1995-04
6 CHEN Xin~1,WANG Bao-li~(1*),QU Dong~(2,3),ZHANG Yan~1(1 College of Life Sciences,Northwest Sci-Tech of Agricultrue and Forestry University,Yangling,Shaanxi 712100,China;2 College of Resources and Environment,Northwest Sci-Tech of Agricultrue and Forestry University,Yangling,Shaanxi 712100,China;3 State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau,Yangling,Shaanxi 712100,China);Establishment of the Semi-quantitative RT-PCR System of Sulphur Transporter Genes in Wheat[J];Acta Botanica Boreali-Occidentalia Sinica;2006-02
7 LUO Yu-wei1,XIE Wei-hua2,MA Kai3 (1 College of Horticulture,Jinling Institute of Technology,Nanjing 210038,China;2 Organic Food Development Center of State Environment Protection Administration,Nanjing 210042 China;3 College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China);Correlation between Endogenous Hormones Contents and Flower Bud Differentiation Stage of Ficus carica L.[J];Acta Botanica Boreali-Occidentalia Sinica;2007-07
8 YUAN Zu-li,LIU Xiu-min,LI Hua-xin(College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China);Review on the Relationship between Polyamines and Environmental Stress[J];Acta Botanica Boreali-Occidentalia Sinica;2008-09
9 YAN Feng1,ZHU Chuan-shu2,PANG Bao-ping1*(1 College of Agriculture,Inner Mongolia Agricultural University,Huhhot 010019,China;2 Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);Advance in Research of Plant Receptor-like Protein Kinases[J];Acta Botanica Boreali-Occidentalia Sinica;2009-04
10 TANGKexuan1,KAI Guoyin1,3,ZHANGLei1,2, PANZhen2, ZHAO Lingxia1, SUN Xiaofen1, ZHENGJingui3of Life Sciences, Fudan University,Shanghai 200433,China; 2.SchoolofPharmacy,SecondMilitary Medical University,Shanghai 200433,China;3.Biotechnology Center, Fujian Agricultural and Forest University, Fuzhou 350002,China);Advancesin RACE and Its Application in Plant Gene Cloning[J];Journal of Fudan University;2002-06
【Secondary References】
Chinese Journal Full-text Database 8 Hits
1 LI Shu-feng1,ZHANG Na1,CHEN Yu-ting2,YANG Wen-xiang1,LIU Da-qun1(1.Biological Control Center of Plant Diseases and Pests of Hebei Province /College of Plant Protection, Agricultural University of Hebei,Baoding 071001,China;2.Shanghai Entry-exit Inspection and Quarantine Bureau,Shanghai 201200,China);Cloning and characterization of receptor-like protein TaRLP19 cDNA from wheat[J];Journal of Agricultural University of Hebei;2012-03
2 WANG Ri-sheng1,2,ZHANG Man1,LI You2,3,HUANG Feng-chan1,LIU Wen-jun1,GUO Xiao-yan4 (1 Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China;2 Guangxi Crop Genetic Improvement and Biotechnology Lab,Nanning 530007,Guangxi,China;3 Agricultural College,Guangxi University,Nanning 530004,Guangxi,China;4 Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China);ESTs Analysis of Suppression Subtractive Hybridization Library from Plant Resistance to Disease and Insect Pest[J];Acta Botanica Boreali-Occidentalia Sinica;2012-02
3 Jiang Cong Song Jialiang Huang Ruifang Huang Minren Xu Li'an * Nanjing Forestry University, Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing, 210037;Molecular Cloning and Expression Analysis of a Class V Chitinase Gene from Populus[J];Molecular Plant Breeding;2013-02
4 WANG Fang(Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences,Qiqihar,Heilongjiang 161006);Application of the Suppression Subtractive Hybridization Technique in Plant-Diseases[J];Heilongjiang Agricultural Sciences;2011-11
5 MA Zong-bin1,YAN Gen-tu2,LIU Gui-zhen3,HUO Xiao-ni3,JIA Wen-hua3(1.College of Agronomy,Henan Agricultural University,Zhengzhou 450002,China; 2.Cotton Research Institute,Chinese Academy of Agricultural Sciences,Anyang 455000,China; 3.Seed Administration Station of Henan Province,Zhengzhou 450003,China);Advances in Control Techniques of Cotton Verticillium Wilt[J];Journal of Henan Agricultural Sciences;2012-02
6 TANG Juan1,2,LIN Jing2,WANG Zhong-hua2,CHANG You-hong2(1.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Horticulture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China);Molecular cloning and expression analysis of phosphatidylcholine transfer protein gene in pear[J];Jiangsu Journal of Agricultural Sciences;2012-03
7 WANG Min1,JIAO Rui2,XING Xiao-ping1,FANG Wei-ping3,LI Hong-lian1(1.College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China;2.Life Science Research Center,Henan Agricultural University,Zhengzhou 450002,China;3.Economic Crop Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China);The Molecular Mechanism of Pathogenicity for Verticillium dahliae Kleb[J];Cotton Science;2011-03
8 ZHANG Shuai,CUI Jin-jie,L Li-min,WANG Chun-yi,LUO Jun-yu (State Key Laboratory of Cotton Biology / Cotton Research Institute,Chinese Academy of Agricultural Sciences,Anyang,Henan 455000,China);Response of Three Resistance Genes to Damage Caused by Aphis gossypii(Hemiptera: Aphididae) in Cotton[J];Cotton Science;2012-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Chuan-liang,WU Zhi-xia,ZHANG Chao-jun,LI Feng-lian,WANG Yu-fen,LI Fu-guang (Chinese Cotton Research Institute, Chinese Agricultural Academy of Sciences; Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture,Anyang,Henan 455112,China);Study on large-scale and high efficient transformation system mediated by Agrobacterium tumefaciens of cotton[J];Acta Botanica Boreali-occidentalia Sinica;2004-05
2 Qin Yonghua Liu Jinyuan* Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084;Cotton Tissue Culture and Plant Regeneration[J];Molecular Plant Breeding;2006-04
3 Shi Xiangyu\ Gao Guoqiang\ Su Xuehe\ Zhu Doubei\ Chen Xiao (Institute for Application of Atomic Energy, Shandong Academy of Agriculture Sciences, Jinan\ 250100) Cui Ping\= (Shandong Education College, Jinan\ 250013);STUDIES IN GETTING HYBRIDS PLANTS OF INTERSPECIFIC\=HYBRIDIZATION THROUGH OVULE CULTURE IN VITRO[J];(拉丁文)ACTA AGRICULTURAE NUCLEATAE SINICA;1999-04
4 Li Shezeng 1) Ma Ping 1) Liu Xingzhong 2) H. C. Huang 3) Chen Xinhua 1) ( 1) Instistut of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000,China; 2) Institue of Biological Contro;Biological Control of Cotton Verticillium Wilt by Antagonistic Bacteria[J];Journal of Huazhong Agricultural;2001-05
5 Liu Guizhen 1 Lan Haiyan 2 Tian Yingchuan 3 Li Shucheng 4 Le Jinhua 5 Wang Lanlan 1 Chen Zhenghua 1 1Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101 2Institute of Industrial Crops, Xinjiang Academy of Agri;Studies on Verticillium and Fusarium Wilt Resistant Cotton Plants with Trangenosis by Laser Microbeam Puncture[J];CHINESE JOURNAL OF LASERS;2000-03
6 LIN Ling1,CHEN Huaigu1,LIU Lei2,ZHANG Aixiang1,WANG Yuzhong1,SHI Jianrong1(1.Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing*"210014,China;*"2.Department of Plant Protection,Agricultural College,Yangzhou University,Yangzhou*"225009,China);Screening of Antagonistic Bacteria against Sharp Eyespot of Wheat and Assay of Their Biological Activity[J];Jiangsu Journal of Agricultural Sciences;2003-03
7 ZHOU Zhao-hua, DAI xin, PAN Jia-ju, ZHANG Tian-zhen, CHEN Zhao-xia ( Department of Agronomy, Nanjing Agricultural University, Nanjing 210095; Agro- reseach Station of Xushi Town,, Changshu City, Jiangsu 215535,China );Resistance Analysis of Upland Cotton Varieties against Multi Verticillium dahliae Isolates[J];Acta Gossypii Sinica;2001-01
8 JIAN Gui liang , SUN Wen ji , MA Cun (Institute of Plant Protection,CAAS,Beijing 100094, China);A New Method for Identifing the Verticillium Wilt Resistance: Sprinkle the Strain Suspention on Root in Non bottom Plastic Film Bottle[J];Acta Gossypii Sinica;2001-02
9 JIANG Shu li,WANG Xue de (Department of Agronomy,College of Agriculture & Biotechnology,Zhejiang University,Hangzhou,310029,China);Fiber Induction and Development from Cotton Ovules of Five Fiber Mutants in vitro[J];Acta Gossypii Sinica;2002-02
10 WANG Xing fen, MA Zhi ying (College of Agriculture, Agricultural University of Hebei, Baoding 071001, China);A New Method for Identification of Cotton Verticillium Wilt Resistance[J];Acta Gossypii Sinica;2002-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved