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《Cotton Science》 2008-02
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Molecular Cloning and Expression Analysis of Two Chitinase in Upland Cotton

YANG Yu-wen, ZHANG Bao-long, NI Wan-chao, SHEN Xin-lian, ZHANG Xiang-gui, XU Ying-jun(Institute of Agriculture Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)  
ESTs encoding class Ⅲ and classⅣ chitinase respectively were isolated from the SSH library inoculated with Verticillium wilt. Both of them contained full 3’ end and their full open reading frame were obtained by RACE and RT-PCR. As a result, the entire coding region of class Ⅲ and classⅣ chitinase is 1390 bp and 678 bp respectively and encodes a polypeptide of 298 and 226 amino acids. The polypeptide encoded by these two genes shared 71% and 65% homology to their homologous proteins of Arabidopsis. They were designated as Ghachi3 and Ghachi4. All of these two genes have signal peptide and were predicted to be extracellular chitinase. Ghachi3 were abundant in petals, buds and phloem, whereas Ghachi4 only showed an extremely high level in phloem by semi-quantitative RT-PCR. The accumulation of Ghachi4 increased remarkably after treatment of Verticillium wilt and Fusarium wilt in the disease resistant cultivar exclusively. But Ghachi3 was also induced by Verticillium wilt in the susceptible cultivar. In addition, the expression of both the two genes increased remarkably after treatment of ABA. All the results indicated that these two genes might play roles in response to biotic and abiotic stresses.
【Fund】: 江苏省农业科学院科研基金(6110528)
【CateGory Index】: S562
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