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《Cotton Science》 2010-01
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ESTs Analysis of Suppression Subtractive Hybridization Library from Upland Cotton Resistant Cultivar Infected by Verticillium dahliae

ZHANG Chun-ying,WANG Xing-fen,ZHANG Gui-yin,WU Li-qiang,CHI Ji-na,LI Zhi-kun,MA Zhi-ying (Key Laboratory of Crop Germplasm Resources of Hebei/ Agricultural University of Hebei,Baoding,Hebei 071001,China)  
In the study,we screened the suppression subtractive hybridization (SSH) library of resistant upland cotton cultivar,named Jimian20 infected by Verticillium dahliae. More than 400 bp fragments were selected and sequenced from 800 positive clones. The results showed that 203 ESTs were unique. ESTs similarity analysis indicated that 170 ESTs were homologous to the known genes. According to the function of putative proteins,these homologous sequences were related to ten kinds of roles as reported,including metabolism,defense,stress,signal transduction,ribosomal protein,cell structures,cell development,energy,osmotic adjustment,protein synthesis and decomposition and so on. ESTs of unknown function were also a large proportion,ac-counting for 16.26%. Sixty-six ESTs were related to disease-resistance genes,accounting for 33% of all ESTs. Most of them had high similarity to the defense,secondary metabolites,stress and signal transduction. The above results would be helpful to under-stand the molecular mechanisms of Verticillium resistance in cotton.
【Fund】: 国家自然科学基金(30871562);; 河北省自然科学基金重大项目(C2006001034);; 河北省科学技术厅重点项目(08965508D)
【CateGory Index】: S435.621
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