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《Cotton Science》 2011-01
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Molecular Cloning and Expression Analysis of a Receptor-like Protein Kinase Gene in Upland Cotton

YANG Yu-wen,HE Bing,ZHANG Bao-long,NI Wan-chao (Institute of Agribiotechnology,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Jiangsu Agricultural Biology,Nanjing 210014,China)  
The cDNA encoding the full length of a receptor-like protein kinase of cotton was cloned through degenerate primers amplification,genome walking and RT-PCR.Sequence analysis showed that introns were absent in the gene.It contains a 2964 bp open reading frame,encodes a deduced protein of 988 amino acid residues,and shows 60% homology to HAESA-like2,which regulates floral organ abscission in Arabidopsis.This gene was tentatively designated as GRLK5.GRLK5 expresses higher in seed,stem bark,root,and fibre than in other organs.The ABA treatment induce the expression of GRLK5.The over expression vector was constructed by inserting GRLK5 into the pCAMBIA2301,tobacco plants were transformed by co-cultivating leaves method via Agrobacterium mediation.The object gene was verified to have been integrated into the genome of tobacco by PCR.
【Fund】: 转基因生物新品种培育重大专项“转基因抗黄萎病棉花新种质研制”(2009ZX08005-003B)
【CateGory Index】: S562
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