Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Cotton Science》 2011-01
Add to Favorite Get Latest Update

Identification of the Pathogenicity-related Gene DVK1 in Verticillium dahliae

GAO Feng1,PENG Shan1,PENG Xiao-ling1,LI Hui2,LI Guo-ying1 (1.The Key Laboratory of Prevention and Control for Oasis Crop Disease,Shihezi University,Shihezi,Xinjiang 832003,China;2.Department of Plant Pathology,China Agricultural University,Beijing 100193,China)  
In this study,we carried out T-DNA insertional mutagenesis to identify mutants,which are effective in pathogenicity.The highly virulent,defoliating strain was mutated through Agrobacterium tumefaciens-mediated transformation,and 5000 transformants were obtained.Pathogenicity test of randomly selected 1000 transformants found that five mutants lost their virulence on a susceptible cotton cultivar.A new mutant,d1,was unable to cause full disease on cotton.Analysis of the mutation using Thermal Asymmetric Interlaced PCR(TAIL-PCR) confirmed an insertion into a gene DVK1.The full length of DVK1 genomic DNA and cDNA sequences were obtained using TAIL-PCR and RT-PCR methods.DVK1 has an open reading frame of 3325 bp interrupted by two introns with 52 bp and 36 bp,respectively,and putatively encodes a 1079 aa protein.The reduced pathogenic phenotype of d1 was fully complemented by reintroduction of the gene,indicating DVK1 is essential for pathogenicity in V.dahliae.
【Fund】: 农业部基础性工作专项(SB2007FY027)
【CateGory Index】: S435.62
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 WANG Xing fen, MA Zhi ying (College of Agriculture, Agricultural University of Hebei, Baoding 071001, China);A New Method for Identification of Cotton Verticillium Wilt Resistance[J];Acta Gossypii Sinica;2002-04
2 PENG Shan1,2,Lü Xue-lian1,2,GAO Feng1,2,LI Guo-ying1,2,LI Hui1,2(1.Department of Plant Protection,Shihezi University,Shihezi 832003,China;2.Key Oasis Eco-Agriculture Laboratory of Xinjiang,Shihezi University,Shihezi 832003,China);Study on a New Rapid Inoculation Method for Verticillium wilt and Fusarium wilt of Cotton[J];Cotton Science;2008-03
3 MA Ping, H. C. HUANG, LI She-zeng, TANG Wen-hua (Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, China;Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada, T1J 4B1;Department of Plant Pathology, China Agricultural University. Beijing 100094, China);A new inoculation method for Verticillium wilt on cotton and its application in evaluating pathogenesis and host resistance M[J];Acta Phytopathologica Sinica;2004-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Jie1,NI Xiuhong1,GU Zhenfang2(1.Nanhui District Planting Technology Extension Center,Shanghai 201300;2.College of Agriculture and Biology,Shanghai Jiaotong University);Toxicity Test of the Systemic Fungicides to Fusarium oxysporum f. sp. melonis[J];Journal of Changjiang Vegetables;2009-12
2 XUAN Zhao-ling,ZHANG Gui-yin,WANG Xing-fen,MA Zhi-ying(Key Laboratory of Crop Germplasm Resources of Hebei,Agricultural University of Hebei,Baoding 071001,China);Effect of different isolate combinations of Verticillium dahliae Kleb.on pathogenecity[J];Journal of Agricultural University of Hebei;2006-06
3 ZHANG Liang et al(College of Plant Protection,Hebei Agricultural University,Baoding,Hebei 071001);Study on Dynamic Distribution of Verticillium Wilt in Plants of Resistant and Susceptible Cotton Varieties by Using DAS-ELISA Technique[J];Journal of Anhui Agricultural Sciences;2010-17
4 WANG Xue,MA Jun,ZHANG Gui-yin,LI Xi-huan,WANG Xing-fen*,MA Zhi-ying*(Agricultural University of Hebei,Key Laboratory of Crop Germplasm Resources of Hebei,Baoding 071001,China);Proteomic Analysis of Cotton Leaf under Verticillium dahliae Stress[J];Cotton Science;2007-04
5 WANG Xing-fen,TIAN Hai-yan,MA Zhi-ying,ZHANG Gui-yin,XUAN Zhao-ling,WANG Wen-sheng,SUN Yan-xiang(Agricultural University of Hebei,Key Laboratory of Crop Germplasm Resources of Hebei,Baoding 071001,China);SSH Library Construction of Upland Cotton Resistant Cultivar under the Stress of Verticillium dahliae[J];Cotton Science;2008-01
6 PENG Shan1,2,Lü Xue-lian1,2,GAO Feng1,2,LI Guo-ying1,2,LI Hui1,2(1.Department of Plant Protection,Shihezi University,Shihezi 832003,China;2.Key Oasis Eco-Agriculture Laboratory of Xinjiang,Shihezi University,Shihezi 832003,China);Study on a New Rapid Inoculation Method for Verticillium wilt and Fusarium wilt of Cotton[J];Cotton Science;2008-03
7 SANG Xi,YUAN Hong-xia,WANG Zhen-yue,LI Hong-lian,WANG Min*(College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China);Pathogenicity of Verticillium dahliae Cotton Isolates in Henan Province and the Wilting Capability of Their Crude Toxins[J];Cotton Science;2010-04
8 ZHAO Jie1, ZHOU Chao-ying1, GU Zhen-fang2 (1. Nanhui District Agro-technology Extension Center, Shanghai 201300; 2. School of Agricalture and Biology, Shanghai Jiaotong Univ, Shanghai 201101, China);Toxicity of Several Fungicides to Fusarium oxysporum f. sp. melonis[J];Journal of Shanghai Jiaotong University(Agricultural Science);2006-04
9 ZHAO Jie1,ZHUANG Juan-yi2,GU Zhen-fang3(1.Shanghai Nanhui District Planting Technology Extension Center,Shanghai 201300,China;2.Shanghai Qibao High School,Shanghai 201101,China;3.Shanghai Jiaotong University Agriculture College,Shanghai 200240,China);Plant Extracts’Inhibition Effect on Muskmelon Fusarium Wilt and Influence on Germination Tests of Cucumis Melo Seeds[J];Journal of Shanghai Jiaotong University(Agricultural Science);2009-04
10 FAN Wei-wei1,2, MA Chun-hong1, DONG Wen-qi 3, LI Yun-chao1, LIU Zi-hui1, JIA Yin-suo1, GENG Jun-yi4, ZHANG Xiang-yun4 (1Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051; 2Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang 050011; 3Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051; 4Institute of Cotton ,Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051);The Influence of the Verticillium dahliae Kleb Infection on Cotton’s Anti-Enzyme[J];Scientia Agricultura Sinica;2006-12
China Proceedings of conference Full-text Database 2 Hits
1 WANG Xue, WANG Xing-fen, MA Zhi-ying, ZHANG Gui-yin, LI Xi-huan (Agricultural University of Hebei, Key Laboratory of Crop Germplasm Resources of Hebei, Baoding, 071001, China);Proteomic Analysis of Cotton Leaf under Verticillium dahliae Stress[A];[C];2006
2 CAO Gui-yan, LIU Yan-zhen, SUN Chang-jun (Instiute of Industrial Crops, Liaoning Academy of Agriculture, Liaoyang 111000);Evaluation Stotus and coutmeasuresn of Cotton Resistance toVerticllium Wilt[A];[C];2006
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Xingfen;Zhang Guiyin;Li Ruiqi;Ma Zhiying (Shool of Agronomy, Hebei Agtic Univ, Baoding 071001);Inheritance of Resistance to Verticillium wilt in Gossypium hirsutum[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1999-02
2 Dai Guanghui Liu JunfangLi Yanzeng Liu Keming Wei Jiankun;A New Method for Testing Cotton Resistance to Verticillium dahliae[J];Acta Agriculturae Boreali—Sinica;1989-04
3 JIAN Gui liang , SUN Wen ji , MA Cun (Institute of Plant Protection,CAAS,Beijing 100094, China);A New Method for Identifing the Verticillium Wilt Resistance: Sprinkle the Strain Suspention on Root in Non bottom Plastic Film Bottle[J];Acta Gossypii Sinica;2001-02
4 WANG Xing fen, MA Zhi ying (College of Agriculture, Agricultural University of Hebei, Baoding 071001, China);A New Method for Identification of Cotton Verticillium Wilt Resistance[J];Acta Gossypii Sinica;2002-04
5 FANG Hui-yong, ZHANG Gui-yin , MA Zhi- ying (College of Agriculture , Hebei Agriculture U- niversity , Baoding 071001, China);Disease Dynamic and Resistance I-dentification to Verticillium Wilt of Transgenic Cotton[J];Acta Gossypii Sinica;2003-04
6 LI She-zeng, MA Ping, HUANG H C, CHEN Xin-hua (Institute of Plant Protection , Hebei Academy of Agricultural and Forestry Science , Baoding 071000, China;Lethbridge Research Centre, Agriculture and Agri-food, Canada, Lethbridge, Alberta, Canada T1J 4B1);Statistical Basis for Determining Ver- ticillium Wilt Resistance of Cotton Cultivar/Line according to Relative Disease Index[J];Acta Gossypii Sinica;2003-06
7 QI Jun-sheng,LI Huai-fang~*(College of Agronomy and Biotechnology,China Agriculture University,Beijing 100094,China);A New Detection Method of Wilting Induction by Phytotoxin from V.dahliae on Cotton through Leaf Pricking and Spreading[J];Cotton Science;2006-04
8 Ma Zhiying Wang Xingfen Zhang Guiyin Li Xinghong Liu Shuqian Wu Liqiang Liu Zhanguo Hebei Agricultural University, Baoding 071001 Sun Jizhong Liu Jinlan Huazhong Agricultural University, Wuhan 430070;Study on the Pathogenicity of Verticillium dahliae of Cotton in Hebei Province[J];ACTA GOSSYPII SINICA;1997-01
9 MA Cun, JIAN Guiliang, SUN Weiji (Institute of Plant Protection, CAAS, Beijing100094, China);Problem and Countermeasure on Resistance Identification to Verticilium Wilt of Cotton in China[J];ACTA GOSSYPII SINICA;1999-03
10 Pan Jiaju;Zhang Tianzhen;Kuai Benke;Guo Xiaoping and Wang Mi(Dept of Agronomy,Nanjing Agric Univ,Nnjing 210095);STUDIES ON THE INHERITANCE OF RESISTANCE TO VERTICILLIUM DAHLIAE IN COTTON[J];JOURNAL OF NANJING AGRICULTURAL UNIVERSITY;1994-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved