Obtaining Transgenic Cotton Plants with Cowpea Trypsin Inhibitor Gene
Li Yane Chen Zhixian Wu Xia Li Shujun Jiao Gaili Wu Jiahe Fan Xiaoping Meng Jinhong The Institute of Cotton, Shanxi Academy of Agricultural Sciences, Yuncheng Shan Xi 044000 P. R. China Zhu Zhen Wang Wei Zhu Yu Xu Honglin Xiao Guifang L
It has been successful to transfer the cowpea trypsin inhibitor(CpTI) gene into cotton(Gossypium hirsutum L.) by A.tumefaciens mediated transformation. Hypocotyl segments from 5 day old seedlings were co-cultured with A.tumefaciens containing foreign CpTI gene and Npt Ⅱ gene. Kanamycin resistant calli were obtained by screening on selective medium. Npt Ⅱ positive calli were subcultured on embryo-inducing medium untile embryos developed. Transgenic cotton plantlets were regenerated on differentiation medium from these embryos. It has been confirmed that transgenic cotton offsprings still carrried foreign CpTI gene and marker gene by PCR, Southern blot and Npt Ⅱ assay. Results of bioassay demonstrated that the transgenic cotton lines were with higher resistance to cotton bollworm(Helicoverpa armigera).
【CateGory Index】： Q78,S562