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《Acta Tritical Crops》 2005-02
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Technique of Chromosome Doubling for Haploid of Anther Culture in Spring Wheat

WANG Jing-dong~1, REN Xian~2, ZHANG Xi-yan~1, DONG Jian-li~1, HE Jun~1(1. Biotech Lab., Ningxia Academy of Agricultural Science, Yinchuan, Ningxia 750002, China; 2. The No.2 Ethical College in Northwest China, Yinchuan, Ningxia 750002, China)  
To increase chromosome doubling frequency of haploid in wheat anther culture. The callus and regenerative plants were treated with colchicines as follows: 1) Five (different) (concentration) of colchicine [0.02‰, 0.05‰, 0.1‰, 0.3‰ and 0.5‰] were added in the callus (differentiation) medium; 2) Roots of (regenerative) plant were soaked in 0.2% and 0.05% colchicine (solutions) for 5 hours and 15 hours (respectively). The result showed that the treated callus grew slowly and had poor (regeneration) and survival rate, even some browing calli died. Long time soaking the roots of (regenerative) plant with colchicine (soluion) (resulted) in the death of most plants whatever high or low colchicines concentrations, but short time of colchicine treatment showed a better result. Soaking roots of (regenerative) plant in 0.2% colchicines (solution) for 5 hours average 45.9% of seed setting could be achieved, and it is 32% higher than that of untreated check (13.2%).
【Fund】: 宁夏回族自治区科技攻关项目(2002-009)。
【CateGory Index】: S512.12
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