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《Journal of Triticeae Crops》 2006-04
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A New Scoring Method of SSR Patterns for Wheat

WANG Li-xin~1,ZHAO Chang-ping~1,QIU Jun~2,LI Hong-bo~1,Ge Ling-ling~1,SUN Hui~1,YAO Ji~3 (1.Beijing Engineering and Technique Research Center of Hybrid Wheat,Beijing 100089,China;2.National Agriculture Technique Development Service,Beijing 100026,China;3.College of Life Science and technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China)  
SSR markers are widely used to establish the crop DNA fingerprint.However,the SSR patterns of wheat have more bands and are more complex than other crops.Therefore,it is difficult to score each band with number 1 and 0 when a piece of gel appears with 50~60 lanes.Here,we score each SSR pattern with two digits instead of scoring each band with many pairs of 1and 0.By arranging all of pattern numbers,we can build the fingerprint code for every wheat accession,and thus,each fingerprint code could be easily converted into computer codes.
【Fund】: 北京市农业育种基础研究创新平台子项目(YZPT01-06);; 农业部全国农业技术推广服务中心项目;; 2005年国家冬小麦品种区域试验培训班授课内容之一
【CateGory Index】: S512.1
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