Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Triticeae Crops》 2009-04
Add to Favorite Get Latest Update

HPLC Determination of Catechin,Myricetin,Quercetin and Kaempferol in Barley Grain

YANG Tao1,DUAN Cheng-li1,3,YANG Sheng-chao1,WEN Guo-song1,ZENG Ya-wen2,XIAO Feng-hui1,3(1.Institute of Chinese Medical Materials of YAU,Yunnan Provincial Center for Chinese Medicinal Materials,GAP Technology,Kunming,Yunnan 650201,China;2.Biotechnology and Genetic Resources Institute,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650205,China;3.School of Forestry and Bio-technology,Zhejiang Forestry University,Hangzhou 311300,China)  
In order to determine the contents of barley flavonoids of catechin,myricetin,quercetin and kaempferol,the use of HPLC Separation and Determination of Flavonoids in Barley catechin,myricetin,quercetin,kaempferol content were investigated.The results showed that while the HPLC was carried out on MC-Pack ODS AM-303(5 μm,250 mm_4.6 mm i.d.) column,the mobile phase was distilled water with 0.1% glacial acetic acid(solvent A) and acetonitrile(solvent B),the flow rate was 0.8 mL·min-1;and 10 μL sample of each filtrate was used,the calibration curves were linear in the range of 0.063~2.000 μg(R2=0.9999) for catechin,0.034~1.100 μg(R2=0.9998) for myricetin,0.025~0.800 μg(R2=0.9993) for quercetin and 0.018~0.560 μg(R2=0.9995) for kaempferol,with average recoveries of 96.88%(RSD=1.30%),98.30%(RSD=0.57%),96.29%(RSD=1.20%) and 101.59%(RSD=0.73%),respectively.The assay demonstrated that the method was simple,accurate to quantify the four active components in barley simuniously.
【Fund】: 云南省中药现代化科技专项项目(2002ZY-24);; 农业部公益性行业科研专项项目(nyhyzx07-001-大麦);; 农业部“948”重大项目(2006-G9-5);; 国家科技支撑计划项目(2006BAD02B04)
【CateGory Index】: S512.3
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved