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《Journal of Triticeae Crops》 2013-06
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Effect of Crossover Stress of Low-temperature and Drought on Protein Expression in Subterraneous Stem of Wheat Cultivar Dongnongdongmai 1

YU Jing;HUANG Ru;WANG Jian-fei;XU Chen;LI Huai-wei;CANG Jing;XU Qing-hua;WANG Jun-hong;ZHANG Da;College of Life Sciences,Northeast Agricultural University;  
By using two-dimensional electrophoresis and mass spectrometry techniques,the proteomes in the subterraneous stem of cold-resistant winter wheat Dongnongdongmai 1were comparatively analyzed under the cross adaptation of low temperature and drought.It was observed that within the range of pH 4~7,there were significant differences in the expression levels of 25protein spots in the protein profiles of subterraneous stem between the cross adaptation of low temperature and drought and the low temperature.And the expressions of 9protein spots of the 25were up-regulated,and that of 16were down-regulated.The differentially expressed protein spots were detected by mass spectrometry,and identified by comparing with the retrieval database.The complete peptide mass fingerprinting were obtained,among them,26% were of stress protein,and 38% were of metabolism-related protein.
【Fund】: 东北农业大学科学研究基金项目(2010RCB51);; 黑龙江省教育厅科学技术研究项目(11551067)
【CateGory Index】: S512.1
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