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《Chinese Journal of Aesthetic Medicine》 2008-05
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Effects of recombinanted human angiostatin on vascular endothelial cell

WU Shang-min1,ZHAO Ya-nan1,YANG Li2,WANG Lu2,GUO Shu-zhong2,LIU Yin1,LIU Dan2,LU Kai-hua2(1.Department of Plastic Surgery,Second Afiliated Hospital,Kunming Medical College,Kunming 650101,Yunnan,China.2.The Centre of Plastic Surgery,the Fourth Military Medical University,Xi'an 710032,Shaanxi,China)  
Objective To investigate the function of recombinanted human angiostatin(YH-16) on vascular endothelial cell(EC).Methods ①Different dosage of YH-16 was used in the vascular endothelial cell,and the effective concentration(10μg/ml) of YH-16 was examined by MTT method.②The test was divided into three groups randomly as YH-16-experiment group,PBS-blank control group and RPMI-1640 medium-positive group.③In the following 24h、48h and 72h interventions,the morphous of EC was observed by photology inverted microscope,the cytoactive was detected by method of MTT and the cell cycle was investigated by flow cytometry(FLC).The expression of VEGF was dectected by real-time fluorescent quantitate polymerase chain reaction(Real-time PCR).Results ECs were necrosis in the effect of YH-16,and many cell debris can be seen under the light microscope.The absorptance was degraded by MTT assay(P0.05),and FLC revealed the percentage of G2 stage was mult(P0.05).The express of VEGF was obviously degraded(P0.05).Conclusion YH-16 can suppress the proliferation of vascular endothelial cell actively and it can suppress the DNA synthesis.Simultaneously,it can inhibit the secreting of VEGF,and suppress the prolifetation of EC,also migration and angiogenesis.
【CateGory Index】: R96
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