The in vivo study of viola yedoensis makino decoction regulating the function of Murine peritoneal macrophage
LI Haitao,YANG Lin, ZHANG GuangLing,et al (Cardiology Department, Hospital Affiliated to North China Coal Medical College, Tangshan 063000)
Objective In order to study immuno-regulation mechanism of Viola yedoensis.Method 0.5mL/d decoction of Viola yedoensis Makino(0.5g/mL)was through perfusion into the stomach of C57 mice while the same amount of distilled water was given in the control mice. After 30 days,Peritoneal macrophages were collected in the two groups of mice to observe the influences of V. yedoensis Makino on murine M’s phagocytosis and the ability of secreting TNF-α The test of M’s phagocytosis and activity of TNF-α were detected in the supernatant of LPS-stimulated macrophage using sensitive cell line L929 by MTT method. Results It was showed that in vivo Viola yedoensis Makino can lower M’s phagocytosis and the production of TNF-α secreted by macrophage. Conclusion The findings suggest that Viola yedoensis Makino can control immune function by lower the ability of M’s phagocytosis and amount TNF-α production which reduce the releaseing of inflammatory mediator by macrophage.
【CateGory Index】： R285