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《Immunological Journal》 1989-02
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Preparation and Determination of the anti-Thy-1, anti-L3T4 and anti-Lyt-2 McAb

Lui ping Department of Immunology,Beijing Medical University,Beijing  
Cytotoxicity assay, indirect immunofluorescence, fluorescence-activated cell sorter and lymphocyte transformation inhibition test were used to determine the titration and Specificity of the anti-Thy-1, anti-L3T4 and anti-Lyt-2 McAbs secreted by 20-10-5S, GK 1.5, 53-6.72 cell lines. It was found that anti-Thy-1 McAb combines with 95% of thymus cells and 25% of spleen cells; anti-L3T4 and anti-Lyt-2 bind with 80% and 90% of thymus cells, 20% and 10% of spleen cells. Anti-Thy-1 and anti-L3T4 McAbs plus complement can completely block the responce of spleen cell to ConA stimulation while the responce to LPS was normal. Anti-Lyt-2 McAb blocks the responce of the spleen cell neither to ConA nor to LPS. All of the results were conformed with previous reports except for the result of the cytotoxicity assay of the anti-L3T4 and anti-Lyt-2 McAb which were not reported.
【Key Words】： Thy-l L3T4 Lyt-2 McAbs
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