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《Immunological Journal》 1991-01
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Labelling of Tumor Target Cells with ~(125)I-UdR Isotope and its Application to Detection of LAK Cell Activity

Li Denghua & Cui Zhengyan (Department of Immunology, Shangdong Academy of Medical Science, Jinan 250001)  
This paper reported the labelling of tumor target cell with 125I-UdR isotope and its use in detecting the LAK cell activity. The results showed that the longer the labelling time in a period time and the higher the 125I-UdR and/or FUdR concentration, the higher, the 125I-UdR labelling rate of tumor target cells. The spontaneous release was increasing with the effector and target reaction time. We dected the Lymphokine-activated killer (LAK) cell activity using this method and got better results. In addition, there were a lot of advantages such as lower spontaneous release, higher labelling rate and specific release, etc.
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