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《Immunological Journal》 1991-04
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Inducement and Production of Tumor Necrosis Factor by Murine Peritoreal Macrophage

Zhang Ming et al (Department of Microbiology, Nanjing Medical College, Nanjing 210029)  
In this study, we investigated the conditions for the production of Tumor Necrosis Factor (TNF) by thioglycollateinduced murine peritoreal macrophage. It was demonstrated that the activity of TNF was the highest in 8 to 30 hours after adding Lipopolysaccharide(LPS) and the adequate dose of LPS was 5 to 10μg/ml. Besides, the adequate density of macrophage was 2x 106/ml. The resident murine peritoreal macrophage could not produce TNF. The most interesting results were that the production of TNF needed the continued presence of LPS and the calf serum in media could inhibit the TNF. production markedly. These consults were useful to further research in TNF and macrophage immunity.
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