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《IMMUNOLOGICAL JOURNAL》 1999-04
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DETECTION OF GM CSFmRNA EXPRESSION WITH DIGOXIGENIN LABELED cDNA PROBE

Wang Yaping,Wang Yong,Zheng Min,Jiang Rong,Li Jing (Dept.of Histology and Embryology,Chongqing University of Medical Sciences,Chonqing 400046)  
Human GM CSF cDNA probe was prepared with pUC18 GM plasmid and labeled with digoxigenin 11 dUTP.By using the techniques of cell culture in vitro and in situ hybridization,it has detected to the GM CSFmRNA expression of human fetal thymocyte,bone marrow stromal cell ,Leukemia cell line(HL 60,K562),human cord blood mononuclear cells and fetal liver tissue.The results indicated that digoxigenin labeled GM CSFcDNA probe can provide the sensitivity and simplicity of handling needed to replace radioactive probe in routine laboratory experiment and diagnostic assays.
【Fund】: 国家自然科学基金
【CateGory Index】: R392-33
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