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《Immunological Journal》 2008-04
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Inhibition of histamine release from sensitized mast cells by antibodies specific for parasite-origina-ted IgE-dependent histamine-releasing factors

CHEN Xiao-xiang, HU Xu-chu, XU Jing, YU Xin-bing (Department of Pathogenic Organisms, Guangzhou Medical College, Guangzhou 510182, China)  
Objective To investigate the effects of antibodies specific for parasite-originated IgE-dependent histamine-releasing factors (HRFs) on histamine release from sensitized mast cells induced by recombinant rat IgE-dependent histamine-releasing factor (rRHRF). Methods Wistar rats were respectively immunized with purified recombinant IgE-dependent HRFs from Schistosoma japonicum or Clonorchis sinensis (rSjHRF or rCsHRF), and then the immunized sera were isolated and the total IgG were purified by affinity chromatography. rRHRF with a final concentration of 75 μg/mL were incubated with purified anti-rCsHRF IgG and anti-rSjHRF IgG (with a serial concentration rates of 3/5, 2/5, 1/5, 0) at 37 ℃ for 30 min, then aliquots of the mast cells obtained from the lungs of OVA-immunized rats were added and the histamine release induced by rRHRF was measured by the spectrofluorometric procedure. Purified total IgG from normal rat was used as control. Results Purified anti-rCsHRF IgG and anti-rSjHRF IgG were obtained and these purified IgG inhibited histamine release from the sensitized rat mast cells induced by rRHRF, while the dose-dependent relationship needs more efforts. Conclusion Antibodies specific for parasite-originated IgE-dependent HRFs could inhibit histamine release from the sensitized rat mast cells induced by rat IgE-dependent HRF, which may be one of the immunological mechanisms in the protecting effect of parasitic infection against allergic diseases and propose a new idea for the prevention and control of allergic diseases.
【Fund】: 国家自然科学基金(30571634);; 教育部博士点基金(20060558002)资助项目
【CateGory Index】: R392
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