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《Immunological Journal》 2015-05
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Effects of albumin overload on C3a-C3aR signaling pathway and IL-17 expression in adriamycin nephropathy mice

WEI Xinyi;XIAO Lei;YANG Baohui;YANG Haiping;LI Qiu;Ministry of Education Key Laboratory of Child Development and Disorders & Key Laboratory of Pediatrics in Chongqing & Chongqing International Science and Technology Cooperation Center for Child Development and Disorders;Department of Nephrology and Immunology,Children's Hospital of Chongqing Medical University;  
Proteinuria is a proposed mechanism of progressive renal failure associated with glomerular disease.This study designed to observe the effects of albumin overload on C3a-C3 a R signaling pathway and IL-17 expression in adriamycin nephropathy(ADR) mice, and initially explore immunological mechanism of albumin overload-caused kidney damage. SPF healthy male Balb/c mice were randomly divided into control group, ADR group, and ADR with bovine serum albumin overload(ADR+BSA) group. All mice were uninephrectomized under general anesthesia 2 weeks before setting up ADR model. At the end of the 6thweek, ADR +BSA group received weekly 5 intraperitoneally injections of low endotoxin BSA at a dose of 10 mg/g body weight for 4 weeks. At the end of 6thand 10 thweek, 24 hours urinary protein, serum biochemical indexes and kidney pathological changes were evaluated. Expression levels of C3 a, C3 a R and TGF-β1 in kidney was measured by real-time PCR and immunohistochemistry; expression of IL-17 in kidney was measured by ELISA and immunohistochemistry. We found that expression levels of C3 a, C3 a R, TGF-β1 and IL-17 in ADR group were higher than those in control group(P 0.05). At the end of 10 thweek, ADR +BSA group appeared proteinuria and BUN increasing(P 0.001).Under electronic microscope, we observed glomerular sclerosis of focal segmental and renal tubular damage.Expression levels of C3 a, C3 a R, TGF-β1 and IL-17 in ADR+BSA group were higher than those in control and ADR group(P 0.05). In conclusion, activation of C3aC3 a R signaling pathway exists in adriamycin nephropathy mice. Albumin overload can produce much proteinuria, which could accelerate the progressive kidney damage. Proteinuria activating theexpression of C3a-C3 a R signaling pathway, TGF-β1 and IL-17 in adriamycin nephropathy mice might be the potential mechanism.
【Fund】: 国家自然科学基金面上项目(81270802;81070563);国家自然科学基金青年科学基金项目(81200520)
【CateGory Index】: R726.9
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2 ZHANG Yan-qin,DING Jie△,WANG Fang,ZHANG Hong-wen(Department of Pediatrics,Peking University First Hospital,Beijing 100034,China);Hereditary kidney diseases in children[J];Journal of Peking University(Health Sciences);2013-02
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4 GUAN Na,ZHANG Hong-wen,DING Jie,WANG Su-xia (Department of Pediatrics,Peking University First Hospital,Beijing 100034,China);Clinical and genetic diagnosis of familial juvenile nephronophthisis[J];Journal of Clinical Pediatrics;2008-04
5 DU Juan,SHEN Ying (Beijing Children's Hospital,Capital Medical University,Beijing 100045,China);The Cox's proportional hazard regression model for chronic renal failure in children[J];Journal of Clinical Pediatrics;2010-04
6 BAI Yu-hua(Affiliated Hospital,Inner Mongolia University for Nationalities,Tongliao 028000,China);Advance in the Treatment of Children's HSPN[J];Journal of Inner Mongolia University for Nationalities(Natural Sciences);2008-05
7 LIU Jun-cheng,HU shou-liang,CHENG Jun-zhang.(Department of Nephrology,Yangtze University first affiliated hospital,Jingzhou 434000,China);Diagnosis and treatment of hypertension in children with chronic kidney disease[J];Nei Mongol Journal of Traditional Chinese Medicine;2012-06
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10 Tania Beatriz Romero-Adrian;Jorymar Leal-Montiel;Gerardo Fernández;Alejandra Valecillo;Graduate Studies in Immunology,Institute of Biological Research, Faculty of Medicine, University of Zulia;Service of Pediatric,Southern General Hospital;Rheumatology and Immunology Service,University Hospital;;Role of cytokines and other factors involved in the Mycobacterium tuberculosis infection[J];世界免疫学杂志(英文版);2015-01
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【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
1 SUN Yanping,CHEN Jinhe,CHEN Xianjun Department of Pediatrics,Xianning Medical College,Xiannig (437100);Protective Mechanism of Fufangdanshen Injection on Ultrapathological Lesions of Kidney Proximal Convoluted Tubular Epithelial Cells in Adriamycin-induced Nephrosis Rats[J];Chinese Journal of Integrated Traditional and Western Nephrology;2003-03
2 ZHANG Wei,WANG Li,HE Yi,et al Department of Nephrology,Affiliated Chengdu Women's and Children's Central Hospital,Chongqing Medical University,Chengdu(610091);The Model of Nephritic Young Rats Induced by Adriamycin[J];Chinese Journal of Integrated Traditional and Western Nephrology;2012-12
3 LIN Zhao-Yu, GAO Xiang* (Model Animal Research Center of Nanjing University, Nanjing 210063, China);Genetic research of mouse[J];Chinese Bulletin of Life Sciences;2006-05
4 WANG Jing-jing1,2,FU Rong2,YE Li-yan1,2,CHEN Xin-min2,YU Zi-hua1,2,REN Rong-na2,HUANG Juan2,HUANG Jun-jing2(1.Department of Pediatrics,Fuzhou Clinical Medical College,Fujian Medical University;2.Department of Pediatrics,Fuzhou General Hospital of Nanjing Command,Fuzhou 350025,Fujian Province,China);Genetic Variations of NPHS2 and WT1 Genes in 6 Children with Chronic Renal Failure in Chinese Han Ethnic Group[J];Journal of Applied Clinical Pediatrics;2009-05
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6 ZHANG Yong1,ZHANG Bei1,NING Hua-ying1,GUO Yun-liang1,XING Guang-qun2,SHEN Wei1(1.Qingdao University Medical College;2.the Affiliated Hospital of Medical College, Qingdao University,Qingdao 266003,China);Establishment of a rat model of nephrosis induced by single tail vein injection of doxorubicin[J];Acta Laboratorium Animalis Scientia Sinica;2013-01
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