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《IMMUNOLOGICAL JOURNAL》 1994-01
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Determination of IL-2 and Cytotoxicity of Killer Cells in MTT Colorimetry

Zhu Huifen; Zhang Yue; Wang Xiaolin; Shao Jingfang ;Shen Guanxin (Laboratory of Immunology, Reseach Center of Experinentat Medicine,Tongji Medical University,Wuhan 430030)  
The activity of interleukine 2(IL-2) in culture supernatants of lymphokine-activated killer(LAK)cellsand tumor infiltrating lymphocytes (TIL)as well as cytotoxicity of LAK cells on cultured leukemic cells were detected by MTT colorimetry. The results showed that high activity of IL-2 in culture supernatants of LAK and TIL cells was found as well as it could be used for culture of IL-2 edpendent cell lines and clinic experiment. The significant cytotoxicity of LAK cells on leukemic cell lines could be found in vitro ,which was consistent with the ratio of effector cells to target cells. The number of living leukemic cells is consistently related with the concentration of formazan metabolite of MTT. It suggested that the numbers of living cells and cytotoxicity of LAK cells could be correctly estimated by the determina-tion of OD value.
【Key Words】： IL-2. LAK cells. MTT colorimetry. Leukemic cells. Cytotoxicity
【CateGory Index】： R392-33
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