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《Journal of Nanchang University(Engineering & Technology)》 2001-04
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The Continuous Production of Galacto-oligosaccharide by Immobilized Enzyme

WANG Xiao-lan 1,WANG Bai-yang 2,CAI You-liang 3 (1.School of Life Science and Food Engineering,Nanchang University,Nanchang 330047,China; 2.School of Civil Engineering,Nanchang University,Nanchang 330029,China; 3.Nanchang Jisheng Pharmaceutical Fact  
galactosidase from aspergillus oryzae was immobilized for continuous production of galacto-oligosaccharides(GOS) in fibrous bed reactor.The effect of substrate concentration, pH,reaction temperature and retention time on production of GOS was studied.The optimal reaction condition was determined,substrate concentration was 400 g/L;reaction temperature was 50 ℃;pH was 6.0;residience time was 40 min.After 36 h,D-galactose was fed,the product yield increased from 39.6% to 66.2%.The continuous operation was stable for 96 h.
【Fund】: 南昌大学基础理论基金资助项目
【CateGory Index】: TQ28
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